NRC3的子功能化改变了尼古西亚NRC网络的遗传结构
Ching-Yi Huang1, Yu-Seng Huang1, Yu Sugihara2,3
1Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
PLoS genetics
|September 12, 2024
概括
植物免疫受体,核酸结合域和富含白素的重复蛋白 (NLR) 进化了新的兼容性功能. 尼科蒂亚纳植物中的NRC3变异显示了改变的相互作用,影响了病原体的耐药性.
科学领域:
- 植物免疫力 植物免疫力
- 分子进化分子进化
- 遗传学 遗传学是一种遗传学.
背景情况:
- 核酸结合域和富含白的重复蛋白 (NLR) 对于植物和动物的免疫是至关重要的.
- 在植物中,传感器NLRs激活辅助NLRs (细胞死亡所需的NLR,NRC) 以形成免疫反应的抵抗体复合体.
- 塑造传感器NLR和NRC助手之间复杂的基因兼容性网络的进化途径在很大程度上是未知的.
研究的目的:
- 为了研究导致复杂的NRC网络架构的分子进化事件在solanaceous植物.
- 了解NRC3变种在Solanum和Nicotiana分歧后如何演变子功能化和改变传感器辅助器兼容性.
- 在NRC网络中确定传感器辅助器兼容性的关键分子决定因素.
主要方法:
- 分析自然NRC3变异的单植物,形成三个不同的基因组,基于与传感器NLR Rpi-blb2.2.的兼容性.
- 祖先序列重建以推断进化历史并识别关键氨基酸残留物.
- 自然和模拟NRC3变体的特征,包括它们与Rpi-blb2的相互作用,复合体的形成,以及对Phytophthora infestans的耐药性.
主要成果:
- 在Solanum/Nicotiana分歧之后,Solanaceous NRC3变种经历了次功能化,在Nicotiana中修改了NRC网络的遗传架构.
- 六个关键氨基酸位于NRC3同分体的多个表面上,被确定为Rpi-blb2兼容性至关重要的.
- 与Rpi-blb2兼容的NRC3变种形成了与膜相关的复合体,并提供了对Phytophthora infestans的抵抗力.
结论:
- 在NRC等位基因的突变驱动子功能化,导致改变传感器辅助器的兼容性.
- 这种进化过程有助于增加NRC免疫网络的复杂性.
- 了解这些机制,可以了解植物免疫受体网络的演变.
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