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相关概念视频

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

298
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
298
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

330
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
330
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

318
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
318
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

416
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
416

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A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
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表面增强传输拉曼光谱:复杂矩阵中杂质分析的定量性能.

Julie Horne1, Pierre Beckers1, Pierre-Yves Sacré2

  • 1University of Liege (ULiege), CIRM, ViBra-Sante hub, Laboratory of Pharmaceutical Analytical Chemistry, Department of Pharmacy, Liege, Belgium.

Journal of pharmaceutical and biomedical analysis
|September 12, 2024
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概括

传输表面增强拉曼光谱 (SETRS) 提供了一种可行的方法来分析制药产品中的杂质. 这种技术在复杂矩阵中证明了可靠的量化,为先进的分析应用铺平了道路.

关键词:
分析性能的表现.测试 测试 测试复杂矩阵是一个复杂矩阵.不纯洁性 不纯洁性表面增强传输拉曼光谱法 (SETRS)基于总错误风险的方法.

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科学领域:

  • 分析化学 分析化学
  • 频谱学是一种光谱学.
  • 材料科学 材料科学 材料科学

背景情况:

  • 表面增强拉曼光谱 (SERS) 是一种强大的分子检测技术.
  • 传统的SERS通常使用反向散射检测模式.
  • 探索像传输这样的替代检测模式可以增强分析能力.

研究的目的:

  • 调查和验证用于SERS分析 (SETRS) 的传输检测模式.
  • 在SERS中比较传输与反向散射检测的性能.
  • 评估SETRS的分析性能,用于药品中的定量杂质分析.

主要方法:

  • 研究了使用传输检测模式 (SETRS) 的表面增强拉曼光谱 (SERS).
  • 为了SERS的可行性,比较了传输和反向散射检测模式.
  • 研究了样本体积,采集光学,激光束大小和激光功率对SERS信号的影响.
  • 使用SETRS和基于总错误风险的方法,在药品中量化4-氨基醇杂质.
  • 采用单变量 (二次回归) 和多变量 (部分最小平方回归) 的光谱分析方法.

主要成果:

  • 证明了使用传输检测模式 (SETRS) 进行SERS分析的可行性.
  • 确定了影响SERS信号强度在传输模式中的关键参数.
  • 通过SETRS.成功量化了一种商用药品中的杂质 (4-氨基).
  • 通过使用多个批次,日期和操作员来展示SETRS的稳定性.
  • 部分最小平方回归为光谱分析提供了强大的多变量方法.

结论:

  • SETRS是一种可行且有效的方法,用于复杂矩阵中杂质的定量分析.
  • 该研究验证了SETRS用于常规药物分析,为定量应用提供了新的视角.
  • 这项工作突出了SERS中传输检测的潜力,以提高分析性能.