表面增强传输拉曼光谱:复杂矩阵中杂质分析的定量性能
Julie Horne1, Pierre Beckers1, Pierre-Yves Sacré2
1University of Liege (ULiege), CIRM, ViBra-Sante hub, Laboratory of Pharmaceutical Analytical Chemistry, Department of Pharmacy, Liege, Belgium.
Journal of pharmaceutical and biomedical analysis
|September 12, 2024
概括
传输表面增强拉曼光谱 (SETRS) 提供了一种可行的方法来分析制药产品中的杂质. 这种技术在复杂矩阵中证明了可靠的量化,为先进的分析应用铺平了道路.
科学领域:
- 分析化学 分析化学
- 频谱学是一种光谱学.
- 材料科学 材料科学 材料科学
背景情况:
- 表面增强拉曼光谱 (SERS) 是一种强大的分子检测技术.
- 传统的SERS通常使用反向散射检测模式.
- 探索像传输这样的替代检测模式可以增强分析能力.
研究的目的:
- 调查和验证用于SERS分析 (SETRS) 的传输检测模式.
- 在SERS中比较传输与反向散射检测的性能.
- 评估SETRS的分析性能,用于药品中的定量杂质分析.
主要方法:
- 研究了使用传输检测模式 (SETRS) 的表面增强拉曼光谱 (SERS).
- 为了SERS的可行性,比较了传输和反向散射检测模式.
- 研究了样本体积,采集光学,激光束大小和激光功率对SERS信号的影响.
- 使用SETRS和基于总错误风险的方法,在药品中量化4-氨基醇杂质.
- 采用单变量 (二次回归) 和多变量 (部分最小平方回归) 的光谱分析方法.
主要成果:
- 证明了使用传输检测模式 (SETRS) 进行SERS分析的可行性.
- 确定了影响SERS信号强度在传输模式中的关键参数.
- 通过SETRS.成功量化了一种商用药品中的杂质 (4-氨基).
- 通过使用多个批次,日期和操作员来展示SETRS的稳定性.
- 部分最小平方回归为光谱分析提供了强大的多变量方法.
结论:
- SETRS是一种可行且有效的方法,用于复杂矩阵中杂质的定量分析.
- 该研究验证了SETRS用于常规药物分析,为定量应用提供了新的视角.
- 这项工作突出了SERS中传输检测的潜力,以提高分析性能.
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