在体外发酵过程中,玉米耐性粉III和Bifidobacterium adolescentis之间的相互作用
Xinzhu Zhao1, Shuyu Jia1, Haibo Zhao1
1State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, China; College of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, China.
Food chemistry
|September 12, 2024
概括
抗性粉III (RS3),特别是自化脱枝式RS (ADRS),显著增加了Bifidobacterium adolescentis (B. adolescentis) 的生长. 它的粗表面和细菌细胞外氨酶是这种益生菌繁殖和粉降解的关键.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
背景情况:
- 益生菌在调节肠道微生物群的组成和功能方面发挥着至关重要的作用.
- 耐药粉 (RS) 是一种具有潜在益生菌作用的食纤维.
- 了解特定的RS类型与有益的肠道细菌 (如Bifidobacterium adolescentis) 之间的相互作用,对于制定有针对性的营养策略至关重要.
研究的目的:
- 为了研究耐药粉III (RS3) 对Bifidobacterium adolescentis (B. adolescentis) 的增殖的影响.
- 分析RS3在B. adolescentis.发酵之前和之后的结构变化.
- 确定促进B. adolescentis生长的RS3的关键结构特征以及细菌酶在其降解中的作用.
主要方法:
- 制备和表征自化脱枝耐药粉 (ADRS).
- 培养具有ADRS的B.青少年,并测量细菌增殖.
- 在发酵前和后使用原子力显微镜 (AFM) 对ADRS的表面地形分析.
- 酶活性测定用于确定B. adolescentis. 细胞外氨酶的产生.
- 光谱分析 (FTIR) 评估ADRS发酵后的结构变化.
主要成果:
- 与其他RS3形式相比,ADRS对B. adolescentis表现出最显著的增殖效应.
- ADRS的表面粗度 (Ra = 21.90 nm;Rq = 16.00 nm) 被确定为B. adolescentis增殖的一个关键因素.
- B. adolescentis产生了细胞外氨酶,在ADRS发酵过程中观察到的活性最高,表明它在粉降解中的作用.
- 青少年菌的发酵破坏了ADRS的短距离有序结构,FTIR光谱带的显著减少证明了这一点 (R1047厘米为20.74%和R995厘米为30.85%).
结论:
- RS3的结构特征,特别是表面粗度,对于促进B. adolescentis.生长至关重要.
- 由B. adolescentis产生的细胞外氨酶对于RS3的降解至关重要.
- 优化RS3的结构可以增强其益生菌潜力,并支持有益的肠道细菌的繁殖.
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