在共同培养中使用Bifidobacterium animalis ssp. 乳糖与 Lactobacillus helveticus 加快其增长和发酵在牛奶中通过代谢相互作用
Zhi Zhong1, Fei Sun1, Sheng Xu2
1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot 010018, China.
Journal of dairy science
|September 12, 2024
概括
共同培养的乳杆菌 helveticus H9 和生物细菌动物 ssp. 乳糖 益生菌-M8可以增强细菌生长和发酵效率. 这种益生菌协同作用改善了代谢物生产和乳制品质量.
科学领域:
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
- 食品科学 食品科学 食品科学
背景情况:
- 益生菌对肠道健康和发酵食品至关重要.
- 优化共同培养条件可以提高益生菌的有效性和发酵过程.
- 乳杆菌 helveticus 和 生物细菌动物 ssp. 乳腺菌是关键的益生菌物种.
研究的目的:
- 为了研究Lactobacillus helveticus H9和Bifidobacterium animalis ssp.之间的协同作用. 乳糖 益生菌-M8.8. 乳糖 益生菌-M8.
- 使用代谢学分析共同培养期间的代谢变化.
- 了解共同培养如何影响细菌生长,发酵效率和代谢物概况.
主要方法:
- 培养乳杆菌 helveticus H9 和生物细菌动物 ssp. lactis Probio-M8单独和共同培养 (1:1比) 在牛奶中.
- 使用固相微提取气体染色学质谱法 (SPME-GC-MS) 分析发酵牛奶代谢组.
- 量化活性的细菌数量和确定关键代谢物.
主要成果:
- 双菌株发酵显著增加了Bifidobacterium animalis ssp. 的存在. 与单个菌株发酵相比,Lactis Probio-M8的可活性计数增加了2.33倍.
- 共同培养导致发酵牛奶中的代谢物丰富性和多样性更大.
- 在双菌株发酵牛奶中观察到高水平的特定代谢物,包括酸和氨酸.
结论:
- 共同培养的乳杆菌 helveticus H9 和生物细菌动物 ssp. 乳糖 益生菌-M8加速细菌生长和发酵效率.
- 增强的生长可能是由于Lactobacillus helveticus H9的蛋白质分解活性,产生生长因子.
- 研究结果支持在乳制品发酵中使用这些益生菌株,以提高效率和产品质量.
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