以磁性离子液体为基础的化片作为简单的微设备,用于尿路生物标记物的现场分散液体-液体微提取
Raúl González-Martín1, María J Trujillo-Rodríguez2, Mara G Freire3
1Laboratorio de Materiales para Análisis Químico (MAT4LL), Departamento de Química, Unidad Departamental de Química Analítica, Universidad de La Laguna (ULL), 38206, San Cristóbal de La Laguna, Spain; Unidad de Investigación de Bioanalítica y Medio Ambiente, Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias (IUETSP), ULL, 38206, San Cristóbal de La Laguna, Spain.
Analytica chimica acta
|September 12, 2024
概括
这项研究引入了新的固体磁离子液体 (MIL) 泡片,用于现场分散液体-液体微提取 (DLLME). 这些坚固的片子能够有效地,无能量地分析尿液中的生物标志物,提供更好的稳定性和提取效率.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 环境科学 环境科学
背景情况:
- 磁性离子液体 (MIL) 在分散式液体-液体微提取 (DLLME) 中提供磁性分离,消除了离心/过.
- 以光辅助的DLLME增强了提取剂的分散,而无需外部能量.
- 以前的MIL泡片使用粘性,液态的MILs,限制了它们的应用和稳定性.
研究的目的:
- 开发新的,固体的基于MIL的泡片,用于in situ DLLME.
- 为了提高基于MIL的微提取设备的稳定性和强度.
- 为了实现在尿液中的生物标志物的无能量,现场分析.
主要方法:
- 使用固体Ni (II) 基MIL,转化试剂,沸盐 (NaH2PO4,K2CO3) 和Na2SO4.4的沸片的配方.
- 在实地DLLME通过在样本中溶解片激发,诱导MIL转化.
- 具有光和紫外线检测的高性能液态染色学 (HPLC).
主要成果:
- 新的固体MIL平板电脑首次在现场启用了DLLME.
- 该方法实现了低检测极限 (OH-PAHs的10 ng/L,BP的0.60 μg/L).
- 尿液分析的高精度 (RSD <17%) 和准确性 (94%的恢复) 已被证明.
结论:
- 为DLLME开发了第一个稳定,固体的基于MIL的喷片.
- 现场DLLME模式提供了更高的提取效率和稳定性.
- 这种无能源的方法是生物分析的可行现场平台,标志着基于MIL的化片在该领域的首次使用.
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