银纳米颗粒尺寸对生物膜消除的影响
Ghazal Fakeeha1, Sarah AlHarbi2, Sayed Auda3
1Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
International dental journal
|September 12, 2024
概括
与氧化混合的10纳米银纳米颗粒 (AgNPs) 显示出在根除内牙生物膜方面具有优异的抗菌膜疗效. 这种组合显示了与三重抗生素糊相似的结果,表明其作为内药物的潜力.
科学领域:
- 牙科科学 牙科科学
- 纳米技术纳米技术
- 微生物学 微生物学
背景情况:
- 成功的再生性内牙治疗依赖于有效的内消毒.
- 牙内膜生物膜对治疗结果构成重大挑战.
- 研究新型抗菌剂对于改善消毒方案至关重要.
研究的目的:
- 确定银纳米粒子 (AgNPs) 的最佳大小,以在与氧化 (Ca(OH) 2结合时获得最大的抗菌膜疗效.
- 为了评估不同的AgNP大小与Ca(OH)2结合对体外内牙生物膜的疗效.
主要方法:
- 使用电子显微镜和光谱学对AgNP及其混合物的表征.
- 用 Actinomyces naeslundii 和 Fusobacterium nucleatum 准备和注射牙根段以形成生物膜.
- 治疗各种AgNP大小的根段,结合单独的Ca (OH) 2,单独的Ca (OH) 2,盐水和三重抗生素糊 (TAP).
- 对焦激光扫描显微镜分析以量化1周和2周后的细菌活力.
主要成果:
- 10nm AgNPs + 35% Ca(OH) 2混合物在2周后表现出最高的抗菌膜疗效 (99.5%),与TAP (99.2%) 相比.
- 所有测试的AgNP混合物,除了单独的Ca(OH) 2外,在2周后与1周相比,显示出显著改善的抗菌膜疗效.
- 在任何时间点,在2nm和5nmAgNP混合物之间没有观察到有效性的显著差异.
结论:
- 10纳米的AgNPs+35%Ca(OH) 2混合物是一种高效的内运河药物,用于根除内牙生物膜.
- 这种AgNP-Ca(OH) 2组合的疗效与TAP相当,为再生性内牙治疗提供了潜在的替代方案.
- 延长的应用时间通常会提高内药物的抗菌膜疗效.
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