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来自虫堆肥的树叶球微生物组改变了西红 (Solanum lycopersicum, L.) 中的基因表达和调节途径

J Garcia¹, M Moravek¹, T Fish²

¹School of Integrative Plant Science, Cornell University, 135 Plant Science Building, Ithaca, NY, 14850, USA.

Scientific reports

|September 12, 2024

概括

虫料 (虫) 增强了西红植物的生长和营养吸收. RNA测序揭示了杂物改变了植物基因表达,特别是营养物质的转运器,这表明微生物组的好处.

科学领域:

植物科学植物科学
微生物学微生物学
土壤科学土壤科学

背景情况:

堆肥的肠道微生物群,如Eisenia fetida,可能含有有益的促进植物生长的有机体.
虫或虫造物是微生物群落的丰富来源.
了解虫堆肥对植物生理学的影响对于可持续农业至关重要.

研究的目的:

调查源自虫堆肥的根球微生物群对西红 (Solanum lycopersicum, L.) 植物生长和生理学的影响.
为了比较活体混凝土注射剂与消毒混凝土注射剂和对照剂的影响.
为了分析植物基因表达的变化,以响应菌剂注射.

主要方法:

在17周的温室试验中,使用了三种治疗方法:菌剂 (V),消毒的菌剂 (SV) 和对照剂 (C).
评估植物表型,包括地表生物质和叶子同位素组成 (δ15N).
RNA测序用于分析番茄植物中的差异性基因表达.

主要成果:

番茄根球中的细菌群体组成被虫堆肥处理显著改变.
与对照组相比,在V和SV治疗中,地表生物质和叶子 δ15N 显著增强.
在V处理中,RNA测序发现了营养物质载体基因的显著上调,包括一种高亲和度酸盐载体 (Solyc06g074995).

结论:

来自Eisenia fetida虫的树球微生物组可以积极影响番茄植物的生长和生理学.
菌剂接种改变了植物的基因表达,特别是增强了营养吸收途径.
RNA测序是阐明植物对微生物干预的分子反应的宝贵工具.

关键词:

在RNA-Seqq.球微生物组是指球微生物组.番茄 (Solanum lycopersicum L.) 是一种植物,可以生长长长久.虫堆肥是一种虫堆肥.

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