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在被标记的实验小鼠中验证的丸特异性基因素变体H3t蛋白中进行了全面的翻译后修改
Takayuki Kawaguchi1,2, Michihiro Hashimoto3, Reiko Nakagawa4
1Division of Chromatin Regulation, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki, Aichi, 444-8585, Japan.
Scientific reports
|September 12, 2024
概括
新的实验小鼠显示出丸特异性素H3t.
科学领域:
- 表观遗传学和染色体生物学
- 生殖生物学和精子生成
- 分子遗传学和小鼠模型
背景情况:
- 在细胞分化过程中,染色质结构和核细胞组合发生变化.
- 基因组变异及其后翻译修饰对于基因调节至关重要.
- 丸特异性基因素H3t (H3t) 对于男性性伴侣形成至关重要.
研究的目的:
- 为了生成和验证表达FLAG标记H3t的敲入小鼠.
- 为了研究精子生成过程中H3t的体内功能和翻译后修饰.
- 将H3t修改与正规的H3修改进行比较.
主要方法:
- 一代的H3t-FLAG敲入小鼠.
- 丸组织的免疫组织化学分析.
- 基于质谱分析的基因素翻译后修饰的分析.
主要成果:
- H3t-FLAG成功地被纳入丸中的精子和介质细胞.
- 质谱学揭示了H3t-FLAG和正规的H3.3之间的可比的整体翻译后修饰模式.
- 在H3t-FLAG和大量H3之间观察到某些翻译后修改的相对丰度的特定差异.
结论:
- H3t-FLAG敲入小鼠模型是研究活体中基因组变体功能的一个有价值的工具.
- 在精子生成过程中,H3t经历了特定的翻译后修改,这与正规的H3不同.
- 这些发现促进了对男性生殖中的表观遗传调节的理解.
相关概念视频
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:25Spreading of Chromatin Modifications
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Writers
The writer is an enzyme that can...