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相关概念视频

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Overview
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What is Gene Expression?01:36

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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The Central Dogma01:20

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The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
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相关实验视频

Updated: Jun 13, 2025

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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可定制的基因感知和响应,而不改变内源编码序列.

Fabio Caliendo1,2, Elvira Vitu1,2, Junmin Wang3

  • 1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Nature chemical biology
|September 12, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的方法,可以在不改变基因编码序列的情况下将遗传生物传感器集成到细胞中. 这种合成生物学方法增强了用于生物制造和治疗的细胞控制.

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科学领域:

  • 合成生物学 合成生物学
  • 基因工程是一种基因工程.
  • 细胞工程 细胞工程

背景情况:

  • 合成生物学利用遗传电路来控制细胞功能.
  • 通过CRISPR-Cas9将生物传感器集成到内源基因中,可以监测基因表达,但可能会破坏本源调节.
  • 需要一种方法来嵌入生物传感器而不影响基因编码序列.

研究的目的:

  • 开发一种用于将遗传生物传感器集成到内源基因中的新方法,而不会改变它们的编码序列.
  • 通过数学建模,实现生物传感器响应的精确控制和预测.
  • 在CHO-K1细胞中设计一个功能性的细胞应力传感器和执行器系统.

主要方法:

  • 设计了一个系统,将单导向RNA插入内源基因的终结器区域,激活下游电路.
  • 开发了一个数学模型来预测和微调传感器剂量反应.
  • 在CHO-K1细胞中构建了一个细胞应激传感器和执行器,以条件激活抗虫蛋白BCL-2.

主要成果:

  • 成功地将遗传生物传感器集成到内源基因中,而不改变编码序列.
  • 使用数学模型证明了微调和预测传感器剂量反应.
  • 设计了一种功能性细胞应激传感器和执行器,通过激活BCL-2来增强应激下的细胞存活率.

结论:

  • 开发的平台允许将遗传生物传感器集成到内源基因中,通过准终端区域.
  • 这种方法避免了原生基因编码序列的破坏,并允许可预测的生物传感器反应.
  • 工程细胞应力传感器和执行器系统显示了生物制造,细胞命运控制和基于细胞的治疗方法的潜力.