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控制植物干细胞中超氧化离子对DNA脱甲基的控制

Shiwen Wang¹, Min Liu², Dongping Hu¹

¹Ministry of Education Key Laboratory for Cellular Dynamics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Nature chemical biology

|September 12, 2024

概括

超氧化,一种反应性氧物种,在植物干细胞中激活DNA脱甲基酶ROS1. 这种包括ARABIDOPSIS RESPONSE REGULATOR 12在内的氧化还原信号通路对于维持植物干细胞命运至关重要.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
氧化还原信号传递

背景情况:

超氧化 (O2•−) 是一种具有强烈氧化潜力的活性氧物种,存在于所有生物体中.
超氧化物积聚在植物的干细胞系统中,在芽和根茎中维持干细胞种群中发挥作用.
沉默1的DNA脱甲基酶抑制剂 (ROS1) 是参与DNA脱甲基化的关键酶.

研究的目的:

为了研究超氧化物在植物干细胞中的直接目标.
阐明超氧化物影响DNA脱甲基和干细胞命运的机制.
在超氧化物介导路径中识别下游信号组件.

主要方法:

生物化学试验评估超氧化物和ROS1.1之间的相互作用.
分析ROS1活动,特别是其DNA糖酶/酶功能的分析.
在Arabidopsis进行遗传研究,以检查ROS1和ARABIDOPSIS RESPONSE REGULATOR 12在干细胞维护中的作用.

主要成果:

超氧化物直接氧化ROS1内的铁硫 (Fe-S) 集群,激活其DNA糖酶/酶活性.
超氧化物信号传递在很大程度上参与了在阿拉比多普西斯基因组中建立活性DNA脱甲基化的过程.
阿拉比多普西斯反应调节器12在ROS1介导的超氧化物信号的下游运行,以维持干细胞命运.

结论:

超氧化物作为一个信号分子,直接激活植物干细胞中的DNA脱甲基酶ROS1.
这项研究为超氧化物如何调节植物的干细胞提供了机制框架.
已经证明,氧化还原信号传递和DNA脱甲基化相互作用,定义了植物干细胞的命运.

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