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抑制TRPM4会减缓皮层神经元的神经元发生的进展

Denise Riquelme¹, Nicole Juanchuto-Viertel¹, Carlos Álamos¹

¹Department of Biology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago, Chile.

Molecular brain

|September 12, 2024

概括

暂时受体潜力美拉斯4 (TRPM4) 通道对于神经元发育至关重要. 在发育中的神经元中抑制TRPM4会损害神经元生长并改变平衡,突出其在神经元发育中的作用.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

短暂受体潜力美拉斯4 (TRPM4) 是一种参与非刺激细胞功能的离子通道.
神经元中的TRPM4表达在产前开始,但它在神经元发育中的作用,特别是神经元发生,仍然不清楚.

研究的目的:

研究TRPM4在神经元发育中的作用和神经元发育中的平衡.

主要方法:

使用9-Ph抑制TRPM4,并在不同发育阶段 (DIV 0和DIV 2) 的培养神经元中通过shRNA进行遗传抑制.
评估神经元数量,神经元发育进展,以及Cch诱导的细胞内 (Ca2+i) 增加.

主要成果:

在DIV 0的TRPM4抑制降低了神经元数量和发育速度,在第一阶段停止神经元.
在DIV2的TRPM4基因抑制下降了神经元长度.
在DIV 0增强的TRPM4抑制时,Cch诱导的Ca2+i增加,破坏平衡.

结论:

TRPM4在神经元发育的进展中起着重要作用.
通过TRPM4调节在神经元发育的早期阶段至关重要.

关键词:

皮层神经元发育的发展.细胞内的细胞内.神经元生成神经元发生.TRPM4 TRPM4 在线播放

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