相关实验视频

Updated: May 6, 2026

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: November 3, 2011

可扩展的hESC衍生的心血管原生细胞产生功能性心脏血统细胞用于微组织结构

Siamak Rezaeiani^1,2, Malihe Rezaee², Mojtaba Shafaghi²

¹Department of Applied Cell Sciences, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran.

Stem cell research & therapy

|September 12, 2024

概括

可扩展的人类胚胎干细胞衍生的心血管原生细胞 (CPCs) 可以产生和维持大规模的体外心血管研究. 这些CPC自我更新,并分化为功能性心脏系,提供有价值的细胞来源.

科学领域:

干细胞生物学干细胞生物学
心血管研究的心血管研究.
再生医学是一种再生医学.

背景情况:

人类胚胎干细胞衍生的心血管原生细胞 (hESC-CPCs) 对于体外心血管生理学研究至关重要.
为了满足研究需求,hESC-CPC的大规模生产至关重要.
开发有效的培养系统是扩展hESC-CPCs的关键.

研究的目的:

建立一个有效的培养系统,以大规模扩展hESC衍生的CPC.
确保hESC衍生的CPC保持其分化潜力成为功能性心脏血统.
评估这些扩展的CPC在体外心血管研究中的有用性.

主要方法:

使用四个小分子的4天协议,hESCs被分化为CPCs.
在8段以上的段落中,使用3个因子 (bFGF,CHIR,A83-01) 的介质扩展了CPC.
在扩展的CPC上进行了冷保存和功能差异化试验.

主要成果:

经过8个通道后,CPC的>6,000倍扩张得到了实现.
扩展的CPC通过8个通道保持了基因/蛋白质表达和分化能力.
CPCs分化为心肌细胞,内皮细胞,光滑肌细胞和纤维细胞,形成心脏微组织.

结论:

可扩展的hESC衍生的CPC表现出一致的自我更新和分化为功能性心血管系.
这些CPC为体外心血管研究提供了有价值和可复制的细胞来源.
使用CPC衍生细胞开发的3D平台显示了心血管生理学研究的潜力.

关键词:

心血管前代细胞是什么?结冷保存是一种冷保存.不同化的差异化扩张扩张扩张大规模生产的大规模生产.

更多相关视频

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Published on: March 1, 2016

In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells

In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells

Published on: July 3, 2019

相关概念视频

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...