稳定的平台从CO2中进行梅瓦诺酸生物生产.
Marco Garavaglia1, Callum McGregor1,2, Rajesh Reddy Bommareddy1,3
1BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottingham, Nottingham NG7 2RD, U.K.
概括
工程细菌现在稳定地从二氧化碳中产生有价值的产品. 一个新的等离子体系统确保工程Cupriavidus necator H16保持有效的生物生产所需的遗传工具.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 稳定的增值产品生产需要强大的微生物生物催化剂.
- 多拷贝表达等离子体对高滴度生物合成有利,但在长期培养过程中往往不稳定.
- 库普里亚维杜斯 (Cupriavidus necator H16) 可以将二氧化碳转化为产品,但难以保持等离子体的稳定性,限制了工业应用.
研究的目的:
- 设计和实施等离子体成系统,以提高Cupriavidus necator H16.16中的多复制等离子体稳定性.
- 为了从二氧化碳中实现增值化学品的稳定生产,使用工程化C. necator H16.
主要方法:
- 基于基本基因的补充的等离子体成系统的开发.
- 实施一个利用RubisCO缺乏突变体的补充来固定二氧化碳的系统.
- 设计用于生产美酸盐的美酸盐路径操作子 (MvaES).
主要成果:
- 基于RubisCO补充的等离子体成系统成功稳定了C. necator H16.16中的多复制等离子体.
- 使用稳定等离子体来表达甲酸盐路径操作子 (MvaES) 的结果大约为10 g/L的甲酸盐.
- 从二氧化碳中获得大约25%的碳产量,用于从二氧化碳中生产甲酸盐.
结论:
- 等离子体成系统有效地稳定了工业生物工艺中C.necator H16中的多副本等离子体.
- 这项研究证明了C1原料 (CO2) 中C6化合物的最高报告标位和产量.
- 开发的系统增强了C. necator H16作为可持续化学生产的微生物底盘的潜力.
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