开发和验证下一代测序小组,用于针对多种物种的个性化杆菌根除治疗
Byung-Joo Min1, Myung-Eui Seo2, Jung Ho Bae3
1Forensic DNA Division, National Forensic Service Seoul Institute, Seoul, Republic of Korea.
Frontiers in cellular and infection microbiology
|September 13, 2024
概括
以抗生素耐药性和宿主代谢的下一代测序 (NGS) 为指导,针对 Helicobacter pylori 根除的个性化治疗显著改善了根除率. 这种方法优化了对抗生素耐药H. pylori菌株的治疗.
科学领域:
- 基因组学和个性化医学
- 微生物学与传染病的研究
- 药物基因组学 药物基因组学
背景情况:
- 降低Helicobacter pylori根除率是一个越来越令人担忧的问题,主要是由于抗生素耐药性增加.
- 标准化治疗方案无法考虑个体宿主代谢能力,进一步复杂化了根除.
- 个性化治疗策略有望改善H. pylori感染的治疗结果.
研究的目的:
- 开发和验证用于个性化H. pylori根除治疗 (NGS-PHET) 的下一代测序面板.
- 整合H. pylori抗生素耐药性数据与宿主代谢概况,以定制治疗建议.
- 评估个性化治疗在成功根除 H. pylori 的有效性.
主要方法:
- 一个定制的NGS面板 (NGS-PHET) 旨在准H. pylori和人类质子抑制剂 (PPI) 代谢基因 (CYP2C19,CYP3A4) 中的抗生素耐药性区域.
- 图书馆被构建和测序,数据使用基于已知的抗性变体和宿主代谢的定制框架进行分析.
- 建议个性化治疗方案,并根据实际患者根除结果进行验证.
主要成果:
- NGS确定了与H. pylori抗生素耐药性相关的已知和新型变体.
- 确定了影响PPI代谢的宿主遗传变异,包括CYP2C19广泛代谢器和CYP3A4中间代谢器.
- 基于NGS-PHET框架的个性化治疗建议与12名受试者中有9名的成功根除一致.
结论:
- NGS-PHET方法与定制框架相结合,可实现快速有效的H. pylori根除治疗,特别是在抗生素耐药菌株中.
- 这种个性化的方法通过考虑细菌耐药性和宿主药物基因组学来提高治疗疗效.
- 开发的方法对于临床应用和识别新型抗生素耐药性机制非常有价值.
相关概念视频
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
333
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
333
01:28Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
97
Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
97
01:26Peptic Ulcer Disease IV: Management
75
Medical treatment strategies for peptic ulcers encompass various methods. The primary goal of treatment is to diminish gastric acidity and strengthen mucosal defense mechanisms.
The therapeutic approach involves ensuring adequate rest, implementing drug therapy, promoting smoking cessation, making dietary modifications, and emphasizing long-term follow-up care.
Pharmacological management
The prevailing therapy for peptic ulcers involves a combination of managing the patient's current...
The therapeutic approach involves ensuring adequate rest, implementing drug therapy, promoting smoking cessation, making dietary modifications, and emphasizing long-term follow-up care.
Pharmacological management
The prevailing therapy for peptic ulcers involves a combination of managing the patient's current...
75