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以同位素标记的化学选择性探针用于标记,分离和对亚代谢体的全面定量分析

Hongtao Tian¹, Zhizhen Lai¹, Wenjia Zhang²

¹Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing, 100005, China.

|September 13, 2024

概括

这项研究引入了新的化学选择探针和数据分析程序,用于敏感的代谢物概况. 该方法有助于识别已知和新型代谢物,推进疾病生物标志物发现,包括阿尔茨海默病研究.

科学领域:

代谢学代谢学代谢学
生物标志物发现发现
分析化学分析化学

背景情况:

小分子代谢物是疾病诊断和预后的关键生物标志物.
需要高度敏感的方法来准确地分析代谢物.
现有的方法面临着敏感性和定量准确性的挑战.

研究的目的:

开发和验证一种用于全面代谢物分析的新方法.
为了提高代谢物分析的灵敏度和定量准确性.
为了识别像阿尔茨海默氏症这样的疾病的新生物标志物.

主要方法:

合成多化学选择性探针,针对各种功能组 (碳素,酸盐,氧,氨基,醇,碳).
集成磁性固相提取,直角切割,同位素标记和选择性合.
开发一个自制的程序,高分辨率同位素辅助识别和定量 (HRIAI Quant),用于数据处理.

主要成果:

该方法成功地分析了各种小分子代谢物,并将矩阵干扰降到最低.
HRIAI Quant在人血清中处理了33874个离子对,确定了701个已知的新代谢物和1062个潜在的新代谢物.
该方法用于分析阿尔茨海默病模型 (SAMP8和SAMR1) 的大脑区域中的代谢物.

结论:

开发的方法为小分子代谢物分析提供了灵敏而准确的工具.
这种方法为复杂的生物样本中生物标志物发现提供了一种新的策略.
这些发现为阿尔茨海默病研究和其他代谢研究提供了宝贵的新工具.

关键词:

这是阿尔茨海默氏症.化学选择性探针的探针.定量分析量化分析稳定同位素标记的标签部分代谢组的部分代谢组.

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