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通过不同的方法检测碳酶的方法评估.

Nana Gao1,2, Jing Zhou1,2, Ge Li1,2

  • 1The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Polish journal of microbiology
|September 13, 2024
PubMed
概括

基于CRISPR的快速DETECTR准确地识别了细菌中的卡巴酶,超过了一些传统方法. 这一进步对于控制产生碳酶的细菌和指导抗生素治疗至关重要.

关键词:
检测 检测 检测在WSG上,我们可以看到WSG.方法学评估方法的评估.快速检测 快速检测 迅速检测

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科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 传染性疾病 传染性疾病

背景情况:

  • 碳酶产生细菌 (CPB) 构成全球健康威胁.
  • 早期发现CPB和carbapenemases对于感染控制和有效的抗生素治疗至关重要.

研究的目的:

  • 评估DNA内核酶向CRISPR转载记者 (DETECTR) 检测碳烯酶检测方法的有效性.
  • 将DETECTR的性能与使用全基因组测序 (WGS) 作为黄金标准的传统方法 (APB/EDTA,PCR,NG-test Carba 5,GeneXpert Carba-R) 的性能进行比较.

主要方法:

  • 收集并分析了58个CPB菌株.
  • 雇佣的DETECTR,APB/EDTA,PCR,NG-test Carba 5,GeneXpert Carba-R和WGS用于碳烯酶的识别.
  • 评估每个方法的敏感性和特异性.

主要成果:

  • 检测仪对KPC和NDM碳化合物具有100%的灵敏度.
  • 基因Xpert Carba-R也显示了对KPC和NDM的100%灵敏度.
  • NG-测试Carba 5和PCR对NDM具有96.7%的敏感性.
  • APB/EDTA确定了细胞型分类,但没有特定的基因.

结论:

  • DETECTR是一种高度敏感和准确的检测碳烯酶的方法,与GeneXpert Carba-R.R.相似.
  • DETECTR和GeneXpert Carba-R可以识别所有目标卡巴酶,在特定基因检测方面比APB/EDTA,NG-test Carba 5和PCR具有优势.