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Updated: Jun 22, 2026

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

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在扫描传输电子显微镜 (STEM) 中对金属氧化物纳米结构的可解释结构评估 通过持久同质性进行图像

Ryuto Eguchi1,2, Yu Wen1,2, Hideki Abe1,3

  • 1National Institute for Materials Science, 1-2-1 Sengen, Tsukuba 305-0047, Ibaraki, Japan.

Nanomaterials (Basel, Switzerland)
|September 13, 2024
PubMed
概括

对Pt-CeO2纳米结构的持续同质分析揭示了关键的几何特征. 一个新的描述符量化了混乱,使纳米结构模式的可解释分类.

关键词:
金属氧化物纳米结构.持久的同质性 持续的同质性扫描传输电子显微镜 (STEM) 的使用

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Scanning Electron Microscopy

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Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
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