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基于混合基质的pH自缓冲GABA发酵,由莱维拉克托巴西勒斯 (Levilactobacillus brevis) CD081717进行

Lingqin Wang^1,2,3, Mengya Jia^1,2,3, Dandan Gao⁴

¹State Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, 330047, China.

Bioprocess and biosystems engineering

|September 13, 2024

概括

一种新的pH自缓冲策略增强了Levilactobacillus brevis CD0817.通过胺黄油酸 (GABA) 的发酵. 这种方法使用基质混合来保持最佳的酸度,显著改善GABA产量.

科学领域:

生物技术是生物技术.
微生物发酵微生物发酵
生物化学工程生物化学工程

背景情况:

胺黄油酸 (GABA) 是一种具有重大研究兴趣的生物活性物质.
使用单个基质 (L-酸或L-单谷氨酸) 的现有GABA发酵方法存在局限性.
通过微生物发酵优化GABA生产对于其应用至关重要.

研究的目的:

为增强GABA发酵开发和评估pH自动缓冲策略.
使用这种策略,优化Levilactobacillus brevis CD0817的发酵参数.
在更大规模的发酵器中验证该策略的有效性.

主要方法:

设计了一个pH自动缓冲策略,使用L-glutamic酸和单L-glutamate的混合物.
进行了瓶试验,以优化Levilactobacillus brevis CD0817.的发酵介质组成和温度.
优化的参数在10升发酵器中得到验证.

主要成果:

最佳的发酵介质包括特定度的L-glutamic酸,单L-glutamate,葡萄糖,酵母提取物,MnSO4·H2O,在30°C时在80和80之间.
在10升发酵器中,GABA产量在48小时后达到353.1±8.3g/L.
通过pH自缓冲策略,有效地保持了GABA发酵所必需的酸度,pH从4.56升至6.10.

结论:

使用基质混合物的pH自缓冲策略有效地改善了Levilactobacillus brevis CD0817的GABA发酵.
这一策略为增强微生物发酵过程中的GABA生产提供了一个有希望的方法.
该方法有可能在其他GABA发酵研究中得到广泛应用.

关键词:

这种细菌是Levilactobacillus brevis CD081717 的.玛-氨基黄油酸是什么玛-氨基黄油酸单L-谷氨酸盐的使用方法粉末形式的L-谷氨酸酸.在pH自缓冲发酵过程中,自动缓冲发酵.

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