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Updated: Jun 13, 2025

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
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稳定的大肠杆菌β的骨干NMR共振分配

Sam Mahdi1, Socheata Lim1, Irina Bezsonova1

  • 1Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT, 06030, USA.

Biomolecular NMR assignments
|September 13, 2024
PubMed
概括

我们确定了一个关键的细菌DNA复制蛋白,大肠杆菌β和一个变体的NMR共振分配. 这些任务对于研究DNA复制和修复中的蛋白质相互作用和动态是至关重要的.

关键词:
复制DNA复制DNA复制DNA复制DnaN DnaN 在线观看聚三全酶 (Pol III) 是一种全酶.渐进性β的紧固件

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¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

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The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
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When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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