反应性气体集群离子束用于通过二次离子质谱学进行增强药物分析
Matija Lagator1,2, Bilal Patel1, Sadia Sheraz1
1Department of Chemistry, Photon Science Institute, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.
Analytical chemistry
|September 13, 2024
概括
用二氧化碳合水集束束增强了飞行时间二次离子质谱法 (ToF-SIMS) 中的药物分析. 这提高了灵敏度,并减少了矩阵效应,以获得更准确的分子成像和量化.
科学领域:
- 分析化学 分析化学
- 表面科学是一门学科.
- 质谱测量质量谱测量
背景情况:
- 气体集束离子束 (GCIB) 对于飞行时间二次离子质谱 (ToF-SIMS) 分析至关重要.
- 改变载体气体成分会影响GCIB的形成和喷雾特性.
- 了解GCIB是优化ToF-SIMS性能的关键.
研究的目的:
- 研究 (Ar/CO2) n和 (H2O) n GCIBs的形成和组成.
- 评估二氧化碳兴奋剂对ToF-SIMS中二次离子产量的影响.
- 评估CO2化GCIBs对于制药和生物样本分析的有用性.
主要方法:
- 通过改变载体气体成分 (Ar,CO2,H2O) 来形成各种气体集束离子束 (GCIB).
- 在飞行时间二次离子质谱学 (ToF-SIMS) 中应用GCIB.
- 在生物矩阵中分析小制药化合物和药物.
主要成果:
- 将二氧化碳添加到水中的GCIB显著提高了药品的正离子产量.
- 对二氧化碳兴奋剂的负离子模式反应取决于样本.
- 过量的二氧化碳导致二氧化碳集群,降低产量;集群大小至关重要.
- 用二氧化碳合的水集群在正离子模式下减轻矩阵效应,并改善生物样本的量化.
结论:
- 用二氧化碳等反应性气体对水集群进行兴奋剂,为ToF-SIMS提供了显著的优势.
- 优化的GCIB提高了灵敏度,分辨率和定量分析能力.
- 反应集束束对先进的分子成像和分析充满希望,其有效性取决于实验参数和样本类型.
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