针对侵袭性菌感染的非培养基诊断试验的进展
1Public Health Wales Mycology Reference laboratory, University Hospital of Wales, Heath Park, Cardiff, UK.
Current opinion in infectious diseases
|September 13, 2024
概括
对侵入性真菌病的非培养诊断测试正在推进,侧流测试和分子测试显示出有前途. 结合多种诊断方法对于准确诊断和治疗真菌感染至关重要.
科学领域:
- 医学真菌学 医学真菌学
- 传染性疾病 传染性疾病
- 诊断微生物学 诊断微生物学
背景情况:
- 侵袭性真菌病 (IFD) 和肺囊炎肺炎带来了重大的诊断挑战.
- 传统的诊断方法往往缺乏灵敏度和速度.
- 非传统的诊断提供了更早,更准确的检测的潜力.
研究的目的:
- 审查IFD和Pneumocystis肺炎的非传统诊断方法的现状.
- 为了突出菌类检测方面的进步.
- 讨论新型诊断方法在临床实践中的作用.
主要方法:
- 关于真菌感染非培养诊断试验的最新文献的综述.
- 分析新兴诊断技术的性能特征.
- 评估抗原和基于分子的测试的实用性.
主要成果:
- 在开发非培养菌的真菌学测试方面取得了重大进展,包括侧流测试 (LFT) 和B-D-Glucan测试.
- 在分子诊断测试的标准化和发展方面取得的进展.
- 没有单一的测试是完美的; 结合抗原和分子测试是最佳诊断的建议.
结论:
- 结合包括古典方法在内的诊断试验对于确认或排除真菌病至关重要.
- 下一代测序具有未来的潜力,但需要优化和验证.
- 改善诊断测试的准入和简化至关重要,特别是在低收入和中等收入国家.
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