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Updated: Jun 13, 2025

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PRRSV GP5和M的分子间二硫化键促进了VLP的分泌和细胞结合

Xinnuo Lei¹, Yifan Jiang², Wanting Yu³

¹Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics (LFP) & Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, China; Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, China.

Veterinary microbiology

|September 13, 2024

概括

猪生殖和呼吸系统综合征病毒 (PRRSV) GP5和M蛋白之间的相互作用,特别是它们的二硫化键,对于病毒样粒子 (VLP) 分泌和细胞结合至关重要. 这一发现有助于理解PRRSV感染机制.

科学领域:

病毒学病毒学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

猪生殖和呼吸系统综合征病毒 (PRRSV) 对全球猪业产生重大影响.
GP5和M是参与PRRSV复制的关键结构蛋白.
通过GP5和M共同表达形成病毒样颗粒 (VLP) 已知,但机制尚不清楚.

研究的目的:

研究GP5-M相互作用在PRRSVVVLP分泌中的作用.
为了确定GP5-M相互作用对VLP细胞结合的影响.
阐明GP5-M相互作用的结构基础.

主要方法:

在Sf9细胞中使用重组百科病毒对GP5和M的同时表达.
通过传输电子显微镜确认VLP.
使用Bira技术评估GP5-M相互作用.
对VLP分泌和细胞结合的二硫化键破坏的分析.

主要成果:

GP5和M的共同表达产生了VLPs,分泌量由蛋白质表达水平调节.
证实了GP5-M相互作用;N端二硫化键的破坏削弱了相互作用,减少了VLP分泌.
缺少二硫化键取消了与MARC-145细胞结合的VLP.

结论:

在GP5和M之间的分子间二硫化物键对于它们的相互作用至关重要.
这种相互作用对于有效的VLP分泌和细胞结合至关重要.
结果表明GP5和M之间的潜在相互作用地点,提供了对PRRSV病变的洞察力.

关键词:

双硫化物键是一种二硫化物键.猪生殖和呼吸系统综合征病毒 (PRRSV)蛋白质相互作用的相互作用类似病毒的粒子.

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