通过选择性酶性乙醇溶解在糖中丰富EPA和DHA
Junwen Yu1, Yijie Fu1, Xiao Tang1
1Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Food chemistry
|September 13, 2024
概括
这项研究引入了脂酶催化醇化,以以酸 (EPA) 和多可萨赫萨酸 (DHA) 丰富糖. 埃弗萨®转化2.0选择性地生产了DHA丰富的甘油和EPA丰富的乙烯.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 脂质化学 脂质化学
背景情况:
- 埃可萨氨酸 (EPA) 和多可萨氨酸 (DHA) 是重要的omega-3脂肪酸,具有众多的健康益处.
- 含有EPA和DHA的糖化物是营养和制药应用的重要目标.
- 为了将这些特定的脂肪酸集中到糖形式中,高效的方法非常受欢迎.
研究的目的:
- 开发一种有效的脂酶催化醇解方法,用EPA和DHA丰富糖.
- 为了研究脂酶Eversa® Transform 2.0对EPA和DHA的选择性.
- 为了优化反应条件,最大限度地提高糖产品中DHA和EPA的丰富度.
主要方法:
- 使用Eversa® Transform 2.0.0进行脂酶催化醇化.
- 分子对接以验证脂酶的选择性.
- 优化酒精类型和反应条件.
- 在糖产品中脂肪酸成分的分析.
主要成果:
- Eversa® Transform 2.0 显示出显著的选择性,在酒精分解过程中区分DHA和EPA.
- 酒精类型极大地影响了产品中的脂酶选择性和DHA/EPA比率.
- 在最佳的乙醇化条件下,产生了含有12.91%EPA和55.40%DHA的糖,达到79.22%的DHA产量.
- 成功地分离了DHA丰富的甘油和EPA丰富的乙烯 Ester.
结论:
- 脂酶催化醇解是生产富含EPA和DHA甘油的有效策略.
- 对于选择性丰富特定的欧米茄-3脂肪酸,Eversa® Transform 2.0显示出有希望的结果.
- 这种方法允许针对性地制备DHA丰富的糖和EPA丰富的乙烯.
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