查,识别和针对性干预喘的死菌生物标志物
Ling Feng1, Zhenzhen Wu1, Xinyu Jia1
1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Biochemical and biophysical research communications
|September 13, 2024
概括
亡在喘中被激活,四个关键基因 (NRDEG) 被确定为与肺功能下降和炎症相关的诊断标记. Resveratrol和triptolide通过向这些与亡相关的基因,显示出喘治疗的前景.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 细胞亡,一种被编程的细胞死亡途径,与呼吸系统疾病有关.
- 死在喘病原发生的具体作用尚不清楚.
- 这项研究调查了亡标记物和喘管理的治疗点.
研究的目的:
- 在喘中识别与亡相关的差异表达基因 (NRDEG).
- 评估NRDEG在喘中的诊断和功能意义.
- 探索潜在的治疗药物,以NRDEG为喘治疗的目标.
主要方法:
- 机器学习算法 (LASSO,随机森林,SVM-RFE) 用于NRDEG识别.
- 使用外部数据集,西方抹黑和qPCR验证NRDEG表达.
- 通过ROC分析和与临床指标的相关性对临床相关性的评估.
- 免疫细胞透,单细胞RNA测序和通路丰富分析 (GO,KEGG,GSEA).
- 对潜在的治疗剂进行药物选和分子对接.
主要成果:
- 在喘中发现了四种NRDEG (NLRP3,PYCARD,ALOX15,VDAC3);NLRP3,PYCARD,ALOX15上调,VDAC3下调.
- NRDEGs对喘具有诊断价值,并且与肺功能 (FEV1,FVC) 和炎症标志物 (FeNO,吐/血液中小氨基,中性,IgE) 相相关.
- NRDEG与激活的树突细胞,巨细胞和埃索诺菲尔细胞相关;scRNA-seq揭示了与纤维化和炎症相关的通信.
- 途径分析表明NRDEG参与了核糖体功能,氧化酸化和脂肪酸代谢.
结论:
- 死在喘中被激活,NRDEGs作为疾病风险和肺功能的预测剂.
- NRDEGs调节了喘免疫-炎症微环境.
- Resveratrol和triptolide被确定为有前途的治疗候选人,针对喘的NRDEG.
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