碳氧甲基基托二甲葡萄糖/多多巴胺载体有针对性的输送 Bacillus subtilis增强了小鼠的口服利用和肠道殖民
Lulu Chu1, Luyu Xie2, Bingzhi Chen1
1College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
International journal of biological macromolecules
|September 13, 2024
概括
一种新型的双层载体保护益生菌免受恶劣的肠道环境的影响,增强它们的殖民和有益作用. 这项创新改善了肠道微生物群的多样性,并促进了肠道健康.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 益生菌往往难以在胃肠环境中生存,从而限制了它们的治疗潜力.
- 开发有效的输送系统对于提高益生菌的有效性和肠道殖民化至关重要.
- 现有的载体缺乏足够的保护对消化液,胆汁和抗生素.
研究的目的:
- 设计和评估一个适应pH值的,粘合剂双层载体,用于益生菌.
- 为了保护 Bacillus subtilis (B. subtilis) 并改善其肠道殖民和生存.
- 调查载体保护益生菌对肠道微生物群组成和功能的影响.
主要方法:
- 制造一个 Carboxymethyl chitosan 聚甲 聚糖化物 (CMCS-DHG/PDA) 双层载体.
- 使用紫外线光谱和X射线光电子光谱 (XPS) 进行了表征.
- 评估pH响应,胀行为以及对模拟消化液,胆汁和抗生素的保护.
- 评估粘膜粘附,口腔生物可用性和肠道殖民.
- 分析肠道微生物群的多样性,丰富性,组成和短链脂肪酸 (SCFA) 生产.
主要成果:
- CMCS-DHG/PDA载体成功地用聚多巴胺纳米涂层和希夫基共价键封装了B. subtilis.
- 载体表现出显著的pH反应性,在pH 1.2时比pH 7.4.时更多的胀.
- 携带载体保护的B. subtilis在模拟的消化液中显示出增强的生存率,以及对胆汁和抗生素的耐受性.
- 观察到受保护益生菌的异常粘膜粘附,从而改善了口服生物可用性和肠道殖民.
- 用载体保护的B. subtilis显著增强了肠道微生物群的多样性,丰富性和SCFA生产,促进了更健康的肠道环境.
结论:
- 响应pH的粘合性CMCS-DHG/PDA双层载体有效地保护益生菌并增强其肠道殖民.
- 这种新型的载体系统改善了益生菌的生存,粘膜粘附,对肠道微生物群和宿主健康产生有益影响.
- 开发的载体对开发用于胃肠道疾病的先进益生菌疗法具有重大前景.
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