Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Replisome03:01

The Replisome

33.2K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.2K
Replication in Prokaryotes01:32

Replication in Prokaryotes

24.8K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.8K
Homologous Recombination02:31

Homologous Recombination

50.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.3K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.9K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.9K
The DNA Replication Fork01:02

The DNA Replication Fork

35.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Engineering yeast chromosomal telomeres with a bacteriophage system.

Nature communications·2026
Same author

Effect of risperidone treatment on insulin-like growth factor-1 and interleukin-17 in drug naïve first-episode schizophrenia.

Psychiatry research·2021
Same author

Circularly polarized luminescent systems fabricated by Tröger's base derivatives through two different strategies.

Beilstein journal of organic chemistry·2021
Same author

Cerebral Infarction in Immune Thrombotic Thrombocytopenic Purpura Is Associated with Old Age, Hypertension, Smoking, and Anti-ADAMTS13 Ig, But Not with Mortality.

TH open : companion journal to thrombosis and haemostasis·2021
Same author

<sup>1</sup>H, <sup>13</sup>C, and <sup>15</sup>N resonance assignments of reduced apo-WhiB4 from Mycobacterium tuberculosis.

Biomolecular NMR assignments·2021
Same author

The mitochondrial genome of <i>Suillia</i> sp. (Diptera: Heleomyzidae).

Mitochondrial DNA. Part B, Resources·2020

相关实验视频

Updated: Jun 13, 2025

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

8.9K

重建一个强大的细菌复制模块.

Tao Wang1, Fan He2, Ting He2

  • 1Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences/Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, PR China.

Nucleic acids research
|September 13, 2024
PubMed
概括

研究人员通过集群23个基因来设计一种细菌DNA复制模块 (pRC). 这种人工模块提高了DNA合成的效率和稳定性,为合成基因组学提供了潜力.

更多相关视频

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

13.6K
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.5K

相关实验视频

Last Updated: Jun 13, 2025

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

8.9K
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

13.6K
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.5K

科学领域:

  • 分子生物学分子生物学
  • 合成生物学 合成生物学
  • 基因组学就是基因组学.

背景情况:

  • DNA复制对于生命至关重要,需要复杂的机械和调节.
  • 了解和操纵DNA复制对于生物研究和生物技术至关重要.

研究的目的:

  • 通过人工聚类基因重建一个功能性的细菌DNA复制模块 (pRC).
  • 研究将该模块集成到大肠杆菌染色体中对DNA合成效率的影响.
  • 评估复制模块在遗传稳定性和合成基因组构建方面的潜在应用.

主要方法:

  • 人工集群23个DNA复制基因进入大肠杆菌的一个模块 (pRC).
  • 从它们的自然染色体位点连续删除基因.
  • 整合了pRC模块在各种染色体位置,包括在复制起源附近.
  • 构建了一个最小化的模块 (pRC16),具有必不可少的复原体和延长基因.
  • 将该模块集成到染色体外质粒中.

主要成果:

  • 整合pRC提高了DNA合成效率,效率随着模块靠近复制原点而增加.
  • 具有复制模块的菌株显示出加速的复制叉运动和早期启动染色体复制.
  • 最小化的pRC16模块显示了与完整的pRC模块相比的DNA复制效率.
  • 复制模块确保了在不同生长条件下稳健和快速的DNA复制.
  • 将模块集成到等离子体中,提高了它们的遗传稳定性.

结论:

  • DNA复制可以人工重建成功能模块.
  • 这些复制模块提高了染色体DNA复制效率和遗传稳定性.
  • 这些发现表明,在DNA复制工程和合成模块化基因组构造方面有潜在的应用.