Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cross-reactivity00:42

Cross-reactivity

31.0K
Overview
31.0K
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

474
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
474
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

541
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
541
Hybridoma Technology01:31

Hybridoma Technology

14.1K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
14.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Conservation of Human IgSF Proteins Throughout Eukaryotic Evolution.

Genome biology and evolution·2026
Same author

Tumorigenesis and Tumor Microenvironment in Lung Cancer.

Current issues in molecular biology·2026
Same author

Optimization of a sarbecovirus llama nanobody-antigen binding interface via a combined computational and phage display protein engineering approach.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Computational Prediction of Multiple Antigen Epitopes.

bioRxiv : the preprint server for biology·2024
Same author

Assessing the functional impact of protein binding site definition.

Protein science : a publication of the Protein Society·2024
Same author

Optimal selection of suitable templates in protein interface prediction.

Bioinformatics (Oxford, England)·2023

相关实验视频

Updated: Jun 13, 2025

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

14.9K

对多个抗原表位的计算预测.

Rajalakshmi Viswanathan1, Moshe Carroll1, Alexandra Roffe2

  • 1Department of Chemistry and Biochemistry, Yeshiva College, New York, NY 10033, United States.

Bioinformatics (Oxford, England)
|September 13, 2024
PubMed
概括

我们开发了ISPIPab,这是一种用于预测抗原表位的新型计算工具,提高了疫苗设计和诊断的准确性. 这种方法结合了多种方法,以有效地识别已知的和新的表征.

更多相关视频

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.8K
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.4K

相关实验视频

Last Updated: Jun 13, 2025

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

14.9K
Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.8K
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.4K

科学领域:

  • 免疫信息学是指免疫信息学.
  • 计算生物学 计算生物学
  • 生物技术是生物技术.

背景情况:

  • 准确的抗原表位标识对于疫苗设计,诊断和药物开发至关重要.
  • 实验性表位确定是劳动密集型和昂贵的,需要计算解决方案.
  • 现有的计算表位预测工具在准确性和适用性方面存在局限性.

研究的目的:

  • 开发一种改进的计算方法来预测抗原表位.
  • 为了提高表位预测的准确性和范围,超越当前最先进的方法.

主要方法:

  • 介绍了ISPIPab,这是一个新的抗原表位预测程序.
  • 在ISPIPab.中集成两个基于特征的方法和一个基于对接的方法.
  • 与预测算法一起应用等级集群的应用.

主要成果:

  • 与单个组成方法相比,ISPIPab表现出优越的性能.
  • ISPIPab的表现优于其他最先进的表位预测工具.
  • 综合方法成功地确定了已知的表位,并揭示了新的潜在目标.

结论:

  • ISPIPab在计算表位预测方面取得了重大进展.
  • 该工具有助于识别既定和新的表征,以便进一步研究.
  • 这种方法增强了医学应用,如疫苗开发和免疫诊断.