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Jiangdi Li¹, Yating Chen¹, Rui Zhang¹

¹College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China.

Rice (New York, N.Y.)

|September 13, 2024

概括

OsWRKY70 负面调节了大米的防爆性,但增强了对寒冷的耐受性. 失去OsWRKY70会增加对真菌疾病的易感性,同时也会损害寒冷应激反应.

科学领域:

植物分子生物学植物分子生物学
植物病理学植物病理学
植物生理学植物生理学

背景情况:

WRKY家族的转录因子对于植物适应环境挑战至关重要.
OsWRKY70此前已与大米中的草食动物耐药性和非生物应激反应有关.

研究的目的:

调查OsWRKY70在抗真菌病原体的水免疫力中的作用及其对寒冷耐受性的贡献.
阐明OsWRKY70在应激反应中的功能背后的分子机制.

主要方法:

在CRISPR/Cas9基因编辑中生成OsWRKY70淘汰突变体.
用于基因表达分析的RNA测序 (RNA-seq) 和定量实时PCR (qRT-PCR).
半活体染色体免疫沉降 (ChIP) 试验,以确定基因促进物结合.

主要成果:

OsWRKY70淘汰突变体对Magnaporthe oryzae的抗性增强,反应性氧物种 (ROS) 爆发增加和防御基因表达升高.
OsWRKY70可以负面调节JA信号通路组件OsbHLH6.6.
失去OsWRKY70功能会影响寒冷耐受性,导致氧化损伤增加和抗氧化酶活性降低.

结论:

OsWRKY70通过调节防御信号通路,作为水抗爆的负调节剂.
OsWRKY70积极调节寒冷耐受性,可能通过对寒冷敏感基因的表达.
了解OsWRKY70的双重作用为开发抗压性大米品种提供了潜在的战略.

关键词:

防爆性能防爆性能防爆性能防爆性能耐寒性耐寒性耐寒性耐寒性在 OsWRKY70 里面.大米大米大米大米大米转录法规转录法规

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