外体转录载荷和功能相关性与HNSCC患者的存活率.
Joni Yadav1, Apoorva Chaudhary1, Tanya Tripathi1
1Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India.
BMC cancer
|September 13, 2024
概括
来自HPV阳性和HPV阴性头支状细胞癌 (HNSCC) 的外基因组携带不同的转录. 这些改变的转录,包括SGK1和MAD1L1,与HNSCC患者的存活率相关,这表明它们有可能作为生物标志物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 异构体研究 异构体研究
背景情况:
- 人类乳头瘤病毒 (HPV) 状态会影响头部和部状细胞癌 (HNSCC) 的治疗结果.
- 了解HPV阳性和HPV阴性HNSCC之间的分子差异对于个性化医学至关重要.
研究的目的:
- 描述HPV阳性和HPV阴性HNSCC的明显的外体转录基因特征.
- 研究这些差异出口转录 (DET) 与HNSCC患者存活率之间的关联.
主要方法:
- 从HPV阳性 (93VU147T) 和HPV阴性 (OCT-1) HNSCC细胞系中分离出外体.
- 使用Illumina HiSeq X.进行了转录组分析.
- 来自癌症基因组图谱 (TCGA) 的公开数据被用于评估临床相关性.
主要成果:
- 在HPV阳性和HPV阴性外体之间确定了3785个DET.
- 与蛋白质机械,细胞氧化还原和神经系统疾病相关的途径在HPV阳性外体中过度表现.
- 外体中SGK1和MAD1L1的高表达与HNSCC患者的生存率差相关,HPV阴性外体中FADS3,SGK3和TESK2也是如此.
结论:
- 来自HPV阳性和HPV阴性HNSCC细胞的外基因组携带了不同的转录基因载荷.
- 改变的转录显示出临床相关性,与患者的存活率相关,表明作为HNSCC生物标志物的潜力.
- 这些发现突出了HNSCC治疗的潜在治疗目标.
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