隔离,识别和抗菌特征的乳酸酶ibacillus rhamnosus YT
Chengran Guan1, Feng Li1, Peng Yu1
1Key Lab of Dairy Biotechnology and Safety Control, College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.
Foods (Basel, Switzerland)
|September 14, 2024
概括
Lacticaseibacillus rhamnosus YT显示出作为发酵食品的安全抗菌剂的潜力. 它的无细胞超和细胞有效地抑制了格拉姆阳性和格拉姆阴性腐烂细菌,有助于生物保存.
科学领域:
- 食品微生物学 食品微生物学
- 细菌学 细菌学是一门学科.
- 生物技术是生物技术.
背景情况:
- 发酵食品中的致病微生物存在风险.
- 新型抗菌剂对于确保发酵食品安全至关重要.
- 乳酸细菌 (LAB) 产生与食品工业应用的抗菌代谢物.
研究的目的:
- 从乳酸细菌中分离和识别一种安全的抗菌剂.
- 评估隔离菌株及其成分对腐烂细菌的抗微生物活性.
- 探索Lacticaseibacillus rhamnosus YT在发酵食品生物保存方面的潜力.
主要方法:
- 使用16s rDNA测序和形态学分离和识别一种人类衍生的细菌菌株 (YT).
- 准备和测试无细胞超 (CFS-YT) 和细胞 (Cs-YT) 的抗菌活性.
- 抗菌性质的表征,包括光谱,pH稳定性,热稳定性和酶敏感性.
主要成果:
- 这种YT菌株被确定为Lacticaseibacillus rhamnosus.
- CFS-YT表现出增长依赖的,pH敏感的,恒温稳定的和对酶不敏感的抗菌活性.
- 由于热敏,pH稳定和酶敏感的细胞表面物质,Cs-YT显示出广泛的细菌静电活性.
结论:
- 乳酸菌cillus rhamnosus YT显示出显著的抗菌潜力.
- CFS-YT和Cs-YT可用于控制发酵食品中的腐烂细菌.
- 这种菌株为食品工业中的自然生物保存提供了有希望的替代品.
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