结构化三糖醇与最佳的阿拉基酸和docosahexaenoic酸含量婴儿配方开发:一个生物可访问性研究
Luis Vázquez1, Blanca Pardo de Donlebún2, Alejandra Gutiérrez-Guibelalde1
1Department of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera 9, Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, Spain.
这项研究开发了一种生产三糖醇 (TAG) 的最佳方法,其中阿拉基酸 (ARA) 与多可萨赫萨酸 (DHA) 的比例为2:1,用于早产婴儿营养. 酶酸解表明在sn-2位置上具有优异的ARA:DHA丰富性,并提高了生物可访问性.
科学领域:
- 营养生物化学 营养生物化学
- 脂质化学 脂质化学
- 婴儿营养 婴儿营养
背景情况:
- 多不和脂肪酸 (PUFA),特别是酸 (ARA) 和多可萨赫萨酸 (DHA),对于胎儿和幼儿时代的大脑发育至关重要.
- 过早出生的婴儿往往很难从母亲的来源获得足够的ARA和DHA.
- 在三糖醇 (TAG) 中ARA:DHA的2:1重量比被认为是早产婴儿配方奶粉的最佳.
研究的目的:
- 开发和优化生产具有特定2:1 ARA:DHA比率的TAG的方法.
- 通过丰富甘油骨干的sn-2位置来增强ARA和DHA的生物可用性.
- 为了比较开发的TAG与商业婴儿配方奶粉 (FormulaidTM) 在ARA:DHA比率和sn-2成分方面.
主要方法:
- 酵素酸解和混合微藻油 (富含DHA) 和富含ARA的油被用于生产TAG.
- 酵素酸解的优化涉及不同的温度,基质摩尔比率,脂酶度和反应时间.
- 进行了体外消化试验,以评估最终产品的生物可访问性.
主要成果:
- 混合和酶酸解都实现了目标的2:1 ARA:DHA比率.
- 与混合 (1.6) 和FormulaidTM (0.9) 相比,酶酸解显著增强了ARA和DHA (2.3) 的sn-2组成.
- 优化的酸分解过程产生了75.5%的生物可访问性产品,包括单甘油,自由脂肪酸,TAG和二甘油,与前体油相比,显示出更好的消化能力.
结论:
- 使用*Candida antarctica*脂酶的酶酸解是一种可复制,可扩展和有效的方法,用于生产具有最佳2:1 ARA:DHA比率和增强的sn-2定位的TAG.
- 开发的TAG表现出改善的生物可访问性,使其成为早产婴儿有前途的营养补充剂.
- 在该过程中使用的脂酶证明了可重复使用性,增加了该方法的经济可行性.
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