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相关概念视频

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
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使用微波辅助技术提高冷质量

Majid Yousefi Vardanjani1, Nasser Hamdami2, Mohsen Dalvi-Isfahan3

  • 1Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.

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|September 14, 2024
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概括

微波辅助冷通过创建更小的冰晶来改善按纹理. 这种方法显示了减少中结损伤的潜力,在解后提高了它们的质量.

关键词:
能源消耗 能源消耗是指能源的消耗.微波辅助冷 (MAF) 是一种冷技术.这些都是,,.质量属性质量属性是指质量的属性.

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Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
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Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
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Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
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科学领域:

  • 食品科学 食品科学 食品科学
  • 食品工程 食品工程
  • 材料科学 材料科学 材料科学

背景情况:

  • 按 (Agaricus bisporus) 容易受结损伤,影响质地和质量.
  • 传统的冷方法会导致大量的冰晶形成,导致细胞损伤.
  • 需要新的冷技术来保持的质量.

研究的目的:

  • 为了研究微波辅助冷对按品质的影响.
  • 评估不同微波功率水平如何影响冰晶形成和属性.
  • 确定微波辅助冷的潜力,以改善冷的质地.

主要方法:

  • 按在四个微波功率级别 (0,10,20,30%) 上被结.
  • 在解后,评估了包括冰晶大小,棕色指数和纹理特性 (硬度) 在内的质量属性.
  • 分析了微波系统和冰箱之间的功率比.

主要成果:

  • 增加的微波功率导致了更小,更均的冰晶.
  • 的色指数随着微波功率水平的提高而增加.
  • 的硬度随着微波功率的提高而增加,这表明质地特性得到了改善.
  • 微波操作系统与冷器功率的功率比率在最大功率时大约为20%.

结论:

  • 微波辅助冷有效地减少了按中的冰晶体大小.
  • 这种技术有潜力减轻结损伤,导致优越的质地.
  • 这项研究证实了微波辅助冷的可行性,以保持的质量.