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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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转录变种在转录组分析中的重要性

Kevin Vo1, Yashica Sharma1, Anohita Paul1

  • 1Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA.

Cells
|September 14, 2024
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概括
此摘要是机器生成的。

传统的RNA测序 (RNA-Seq) 基因表达分析忽略了转录变异. 分析转录表达 (TE) 揭示了对基因调节的更准确的理解,防止生物研究中的错误解释.

关键词:
有关RNA测序的RNA测序基因的差异表达基因的差异表达.转录变异的差异表达方式.胚胎干细胞是一种胚胎干细胞.转录的变体 转录的变体热囊细胞干细胞的干细胞

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • RNA测序 (RNA-Seq) 是基因表达研究的一个关键技术.
  • 标准RNA-Seq分析汇总了转录,忽略了来自替代拼接或转录开始地点的转录变异.
  • 转录变异可以编码不同的蛋白质或非编码RNA,影响生物功能.

研究的目的:

  • 研究RNA-Seq分析中忽略转录变异的含义.
  • 为了比较基因表达 (GE) 值与转录表达 (TE) 在小鼠干细胞中的转录因子 (TF) 基因.
  • 突出变种级别分析对于准确的生物学解释的重要性.

主要方法:

  • 来自小鼠胚胎干细胞 (ES) 和热囊细胞干细胞 (TS) 的RNA-Seq数据的分析.
  • 对1334个转录因子 (TF) 基因的基因表达 (GE) 值与转录表达 (TE) 的比较.
  • 在基因和转录水平上对差异表达的统计分析,使用折叠变化和错误发现率 (FDR) p-值.

主要成果:

  • 基因表达 (GE) 分析确定了652个在ES和TS细胞之间差异表达的TF基因.
  • 转录表达 (TE) 分析显示,对高调/低调基因的转录变异<20%显示出一致的表达变化.
  • 在GE分析中未被确定为差异表达的基因内,显著比例的转录 (>21%) 被发现在TE水平上被差异表达.

结论:

  • 基因表达 (GE) 值不能准确地代表表达特征,因为不同的转录变异.
  • 将转录变异分析纳入RNA-Seq提供了对基因功能和调控格局的精确理解.
  • 忽视转录变异可能导致生物学和基因调节研究中的错误解释.