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黄金葡萄球菌对二碳酸盐耗尽的压力反应

Elisa Liberini¹, Sook-Ha Fan^1,2, Arnold S Bayer^2,3

¹Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), University of Tübingen, 72076 Tübingen, Germany.

International journal of molecular sciences

|September 14, 2024

概括

黄金葡萄球菌中的二碳酸盐缺乏导致细胞壁显著加厚和改变糖化,导致对lysostaphin和Triton X-100的抗性增加. 这种应激反应会影响细菌的弹性.

科学领域:

微生物学微生物学
细菌生理学细菌生理学
细胞壁生物学细胞壁生物学

背景情况:

二碳酸盐和二氧化碳对于细菌的碳氧化非常重要.
黄金葡萄球菌依赖MpsABC转运器来控制碳度.
mpsABC删除突变体在环境空气中表现出增长受损.

研究的目的:

为了研究在CO2/二碳酸盐缺乏下S. aureus的细胞和分子变化.
了解突变菌株观察到的抗性背后的机制.
分析碳限制对S. aureus细胞壁组成和基因表达的影响.

主要方法:

电子显微镜用于评估细胞壁厚度.
质谱测量用于muropeptide分析.
流细胞测量用于墙壁泰可酸 (WTA) 糖化分析.
进行比较的转录组分析.

主要成果:

在 ΔmpsABC 突变体中,细胞壁的厚度增加了两倍.
突变细胞对lysostaphin和Triton X-100表现出抵抗力.
氨酸被纳入五甘氨酸桥梁,从而产生了对 lysostaphin 的耐药性.
改变的WTA糖化 (少了α,多了β) 解释了Triton X-100的耐药性.
自解素基因 (如sceD) 的上调和细胞壁定蛋白质,分泌蛋白质,载体和毒素的下调.

结论:

二碳酸盐缺乏作用作为一个压力在S. aureus.
这种压力会导致细胞壁组成的显著变化.
全球基因表达被改变,有助于增强性.
这项研究阐明了一种影响细菌生存的新型应激反应机制.

关键词:

在MpsABC中,MpsABC是指MpsABC.黄金葡萄球菌黄金葡萄球菌碳酸二氧化碳运输器运输器细胞壁上的细胞壁.酸甘油可以是酸甘油.转录组 (transcriptome) 是一个转录组.

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