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在山羊基因组中精确插入AttB序列,使用增强的主编辑器

Aicong Li^1,2, Zhenliang Zhu^1,2, Jing Yang^1,2

¹Key Laboratory of Livestock Biology, Northwest A&F University, Yangling 712100, China.

International journal of molecular sciences

|September 14, 2024

概括

研究人员加强了CRISPR/Cas9工具的原始编辑,以实现更高效的基因编辑. 这种改进的系统成功地将基因序列插入到山羊细胞和胚胎中,推进了转基因动物生产.

科学领域:

分子生物学分子生物学
基因编辑技术的技术
生物技术是生物技术.

背景情况:

主编辑提供精确的基因组修改,没有DNA双链断裂.
当前的原始编辑系统面临效率的限制,原因是目标站点,编辑类型和原始绑定站点长度.

研究的目的:

为了提高原始编辑效率并扩大其在基因插入中的应用.
开发一种更有效的原始编辑系统,用于哺乳动物细胞和胚胎中的基因工程.

主要方法:

在pEGRNA的3'终端内嵌了一个RNA基因.
将所有双胞胎主要编辑因子集成到一个单一的等离子体中.
在山羊细胞和胚胎中应用了增强的主要编辑器,以进行有针对性的序列插入.

主要成果:

在主要编辑效率方面实现了高达3.58倍和2.19倍的增强.
成功地将38bp的attB序列插入山羊细胞中的Rosa26和CCR5位点.
通过微注射,在山羊胚胎中展示了11.9%和6.8%的编辑效率.

结论:

修改后的主要编辑系统显示了改进的编辑和转换效率.
这种增强的系统适用于将异物序列插入到原始细胞和胚胎中.
这项研究扩大了用于转基因动物发展的主要编辑的潜力.

关键词:

CCR5 CCR5 的意思是什么?基因的 RNA 基因是 RNA 基因的基因.罗莎26 罗莎26 罗莎26 罗莎一只山羊,一只山羊.主编辑主要编辑.

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