BpfD是一种c-di-GMP效应蛋白,在Shewanella oneidensis中的皮质生物合成中发挥关键作用
Jean-Pierre Poli1,2, Anne Boyeldieu1, Alexandre Lutz1,3
1Aix Marseille Univ, CNRS, BIP, Marseille, France.
International journal of molecular sciences
|September 14, 2024
概括
在Shewanella oneidensis中,Bpf系统对于皮膜生物膜的形成至关重要,与其在表面相关生物膜中的作用不同. 这项研究揭示了一个复杂的循环di-GMP网络控制Shewanella生物膜的发展.
科学领域:
- 微生物学 微生物学
- 细菌生物膜是一种细菌生物膜.
- 信号传导传导是指信号的传导.
背景情况:
- 谢瓦尼拉 (Shewanella oneidensis) 形成皮膜和与表面相关的生物膜.
- 在Pseudomonas fluorescens中,拉普系统通过LapD和LapG调节粘附的Lapa.
- 在S.oneidensis中,Bpf系统是拉普系统的正义系统.
研究的目的:
- 研究Bpf系统在Shewanella oneidensis生物膜形成中的作用.
- 阐明BpfD与循环di-GMP和其他调节蛋白相关的功能.
- 了解控制S. oneidensis生物膜发育的监管网络.
主要方法:
- 对Bpf系统在皮膜和SSA生物膜形成中的作用的遗传分析.
- 生物化学测试以确定BpfD与循环二-GMP的结合.
- 蛋白质与蛋白质相互作用研究涉及BpfD,迪加尼酸环酶 (PdgA,PdgB) 和CheY3.
主要成果:
- Bpf系统对于皮膜形成是必不可少的,但不是SSA生物膜的形成.
- BpfD通过其EAL域结合循环二-GMP,作为c-di-GMP效应剂.
- BpfD与PdgA,PdgB以及响应调节器CheY3.3相互作用.
结论:
- 与拉普系统相比,Bpf系统在S.oneidensis生物膜架构中发挥着独特的作用.
- BpfD作为一个中央枢纽,集成来自迪加尼酸环酸和CheY3.3的信号.
- 在S.oneidensis中,生物膜的形成是由一个广泛的循环二-GMP信号网络调节的.
相关概念视频
01:39Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
01:29Cytoskeletal Proteins in Bacteria
3.3K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.3K
01:26Protein Modifications in the RER
5.1K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.1K
01:11IP3/DAG Signaling Pathway
11.9K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
11.9K