新型剂N-2-HydroxypropylTrimethyl氨基化物奇托对Streptococcus mutans的抗菌活性
Yuan Gao1, Xiaochen Gong1,2, Qicheng Ruan1
1Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou Key Laboratory of Biomedicine and Advanced Dosage Forms, School of Life Sciences, Taizhou University, Taizhou 318000, China.
Molecules (Basel, Switzerland)
|September 14, 2024
概括
N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) 显示出强大的抗菌活性,可以对抗突变性 estreptococcus,这是导致牙损伤的关键原因. 这种天然化合物有效地破坏细菌细胞壁,膜和代谢通路,为传统抗生素提供了有希望的替代品.
科学领域:
- 生物化学 生化学
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 牙损伤 (DC) 是一种普遍的口腔疾病,主要是由Streptococcus mutans (S. mutans) 引起的.
- 对S. mutans的常规抗生素治疗可能会导致不良反应,包括发展抗生素耐药性.
- 一种天然衍生化合物N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) 具有较低的毒性和良好的生物相容性,表明其作为抗菌剂的潜力.
研究的目的:
- 为了研究N-2-HACC对S. mutans.的抗菌疗效.
- 阐明N-2-HACC抗菌作用背后的机制,重点关注细胞壁透性,细胞膜完整性,必需生物分子合成,代谢活性和生物膜形成.
主要方法:
- 最低抑制度 (MIC) 的确定和抑制率的评估.
- 扫描电子显微镜 (SEM) 用于可视化S. mutans的形态变化.
- 酶活性测定 (性酸酶,Na+-K+-ATP酶,酸盐激酶,酸盐脱酶,酸盐脱酶).
- 测量导电性,吸收性和光度,以评估细胞膜损伤和代谢活性.
- 核酸和蛋白质含量的分析,以及对生物膜相关基因的mRNA表达的量化.
主要成果:
- 针对S. mutans,N-2-HACC的MIC为0.625 mg/mL,抑制率为90.01%.
- 在N-2-HACC处理后,S. mutans的SEM显示了显著的表面化.
- N-2-HACC增加了细胞壁透性 (性酸酶活性升高) 和破坏了细胞膜完整性 (在260 nm时增加导电性和吸收性).
- 代谢活性降低,抑制了葡萄糖分解和TCA循环途径 (酶活性降低).
- N-2-HACC降低了核酸和蛋白质含量,干扰了生物膜形成,并降低了与生物膜相关的关键基因的调节.
结论:
- N-2-HACC具有强大的抗菌活性,可以对抗S. mutans.
- 该化合物通过多种机制起作用,包括损伤细胞膜,增加细胞壁的透性,抑制重要的代谢途径 (糖解和TCA循环),并防止生物膜的形成.
- N-2-HACC代表了一种有前途的新型抗菌材料,用于对抗与S. mutans相关的牙损伤.
相关概念视频
01:23Hand hygiene
3.1K
Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
Hand washing...
3.1K
01:23Amines to Sulfonamides: The Hinsberg Test
3.3K
The Hinsberg test is a method to identify primary, secondary and tertiary amines, named after its pioneer, Oscar Hinsberg. Here, amines are treated with benzenesulfonyl chloride, also known as the Hinsberg reagent, in the presence of an excess of aqueous base, followed by acidification. Based on the nature of the amines, different changes are observed.
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing...
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing...
3.3K
01:18Surface Membrane Barriers
1.1K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.1K