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离子液向细菌生物质提取增强的胡卜素提取

Tiago P Silva¹, Luís Alves¹, Francisco Salgado¹

¹LNEG-Laboratório Nacional de Energia e Geologia, IP, Unidade de Bioenergia e Biorrefinarias, Estrada do Paço do Lumiar, 22, 1649-038 Lisboa, Portugal.

Molecules (Basel, Switzerland)

|September 14, 2024

概括

离子液体 (ILs) 显著增强从细菌Gordonia alkanivorans菌株1B.提取的胡卜素. 与传统技术相比,这种新方法提高了264%的效率和产量.

科学领域:

生物技术是生物技术.
自然产品提取提取自然产品
微生物颜料微生物颜料

背景情况:

胡卜素是一种有价值的化合物,具有强烈的色彩和抗氧化特性,来自各种生物.
戈多尼亚食者菌株1B是一种强大的细菌色素生产者,但其耐溶剂性使传统提取复杂化.
离子液体 (ILs) 显示出从植物和微藻中提取胡卜素的前景,但它们在细菌,特别是Gordonia中的应用尚未得到充分探索.

研究的目的:

研究ILs在从细菌生物质中提取胡卜素的有效性.
开发一种优化,高效的提取方法,减少预处理步骤.
为了增强从Gordonia alkanivorans菌株1B.提升胡卜素的恢复.

主要方法:

从G. alkanivorans菌株1B生物质中提取胡卜素的十九种IL和辅溶剂的选.
使用实验设计优化IL#18 (tributyl(ethyl) 二甲基酸盐) 和乙烯酸乙烯 (EAc) 度.
基于IL的新方法与传统的二甲基硫氧化物 (DMSO) 提取方法的比较.

主要成果:

与乙烯酸乙烯 (EAc) 结合的三甲基酸 (IL#18) 显示出优异的胡卜素提取.
确定了最佳条件:50 μL IL#18,1125 μL EAc,以及400 μL的生物质悬浮液 (36 g/L).
与DMSO相比,基于IL的方法增加了264%的胡卜素产量,消除了生物质干燥和降低了提取温度.

结论:

离子液体,特别是IL#18/EAc,为细菌胡卜素提取提供了一个非常有效的策略.
这种方法显著提高了工艺效率,减少了能源消耗,降低了成本和排放.
这些发现支持ILs在工业规模从微生物来源回收胡卜素的潜力.

关键词:

戈尔多尼亚 (Gordonia alkanivorans) 菌株 1B 种胡卜素是一种胡卜素.提取方法的提取方法.绿色方法是一种绿色的方法.离子液体是有离子的液体.

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