使用矩阵辅助激光吸附/电离式质谱成像技术从不同叶类型的Pinelliae树体进行空间代谢分析
Jiemin Wang1,2,3, Xiaowei Han1,2,3, Yuguang Zheng1,2,3
1College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China.
Molecules (Basel, Switzerland)
|September 14, 2024
概括
来自Pinellia ternata的Pinelliae Rhizoma (PR) 显示出基于叶类型的各种代谢物概况. MALDI-MSI确定了关键化合物,揭示了这种传统中医药中的生化差异.
科学领域:
- 植物化学 植物化学
- 代谢学 代谢学 代谢学
- 传统中国医药 传统中国医药
背景情况:
- 皮内利亚树根 (Pinelliae Rhizoma,简称PR) 是中国传统医学的重要草药,具有多样化的药理学作用.
- 皮内利亚特纳塔表现出叶子形态变异 (桃子,竹子,柳树),但根茎化学差异是未知的.
- 了解这些化学变异对于优化PR的治疗应用至关重要.
研究的目的:
- 根据叶子形态,研究Pinelliae Rhizoma中的代谢物在现场的化学组成和空间分布.
- 为了识别潜在的生物标志物,区分PR根茎与不同的Pinellia ternata叶类型.
- 阐明药用植物中特殊代谢物积累的生物化学基础.
主要方法:
- 矩阵辅助激光消化/电离质谱成像 (MALDI-MSI) 用于 in situ 代谢物识别和空间分析.
- 对35种代谢物的分析,包括类化合物,有机酸,氨基酸,黄类化合物,固醇和氨酸.
- 坐标部分最小平方差分分析 (OPLS-DA) 用于根据叶子形态区分根茎样本.
主要成果:
- 在PR根茎组织中观察到不同的二次代谢物的空间分布模式,对应于不同的叶类型.
- 马尔迪-MSI成功地分析了35种代谢物,揭示了显著的生化变异.
- OPLS-DA有效地区分了不同叶形态的根茎.
结论:
- 这项研究提供了第一个基于使用MALDI-MSI的叶子表型的PR的全面代谢概况.
- 确定了五种潜在的差异性生物标志物 (甲基菲奥波戈纳 B,因诺辛,细胞丁,腺素,白/异黄素).
- 这些发现增强了对PR的化学多样性和治疗潜力的理解,将代谢物局部化与植物形态联系起来.
关键词:
在HPLC中使用HPLC.马尔迪-MSI是什么意思在 OPLS-DA 的基础上.皮内利亚 (Pinelliae Rhizomae) 是一种树枝状的植物.其次代谢物二次代谢物.组织分布 组织分布更多相关视频
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