相关实验视频

Updated: Jun 13, 2025

In vitro Monitoring of Extracellular pH in Real-Time
10:11

In vitro Monitoring of Extracellular pH in Real-Time

Published on: June 3, 2021

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监测不同pH值的缓冲溶液的稀释在非常小的体积中,在生理pH以上和以下的不同pH值

Vinayak J Bhat1,2, Daniel Blaschke1, Sahitya V Vegesna1,2

  • 1Leibniz Institute of Photonic Technology, 07745 Jena, Germany.

Sensors (Basel, Switzerland)
|September 14, 2024
PubMed
概括

一个新的阻抗芯片快速而准确地测量了在没有试剂的稀释后的生物缓冲度. 这项技术可以精确监控增强的生物和化学过程.

关键词:
在 PolCarr® 阻抗芯片上.缓冲器 是一个缓冲器.稀释剂的稀释方法是相当于电路建模的同等电路建模阻抗测量的阻抗测量阻抗光谱法阻抗光谱法

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Measuring Phagosome pH by Ratiometric Fluorescence Microscopy14:39

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相关概念视频

Buffer Effectiveness02:19

Buffer Effectiveness

Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
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Calculating pH Changes in a Buffer Solution02:45

Calculating pH Changes in a Buffer Solution

A buffer can prevent a sudden drop or increase in the pH of a solution after the addition of a strong acid or base up to its buffering capacity; however, such addition of a strong acid or base does result in the slight pH change of the solution. The small pH change can be calculated by determining the resulting change in the concentration of buffer components, i.e., a weak acid and its conjugate base or vice versa. The concentrations obtained using these stoichiometric calculations can be used...
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Indicators02:39

Indicators

Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH of a solution, are...
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Titration of a Weak Acid with a Weak Base01:08

Titration of a Weak Acid with a Weak Base

Weak acids and bases do not undergo dissociation completely, and titrations between these two are rarely studied. When such studies are performed, say, for the titration of a weak acid with a weak base, the titration curve plots the change in pH as a function of the volume of base added. Take the titration of acetic acid with ammonia, for instance. During the titration, these two species form ammonium acetate and water, but the pH change is slow and gradual.
As a result, there is no simple...
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Titration Calculations: Strong Acid - Strong Base02:28

Titration Calculations: Strong Acid - Strong Base

Calculating pH for Titration Solutions: Strong Acid/Strong Base
A titration is carried out for 25.00 mL of 0.100 M HCl (strong acid) with 0.100 M of a strong base NaOH. The pH at different volumes of added base solution can be calculated as follows:
(a) Titrant volume = 0 mL. The solution pH is due to the acid ionization of HCl. Because this is a strong acid, the ionization is complete and the hydronium ion molarity is 0.100 M. The pH of the solution is then:
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Buffers: Overview01:30

Buffers: Overview

Buffers play a crucial role in stabilizing the pH of a solution by mitigating the effects of small amounts of added acid or base. They consist of a weak acid and its conjugate base or a weak base and its conjugate acid. A solution of acetic acid and sodium acetate is an example of a buffer that consists of a weak acid and its salt: CH3COOH (aq) + CH3COONa (aq). An example of a buffer that consists of a weak base and its salt is a solution of ammonia and ammonium chloride: NH3 (aq) + NH4Cl (aq).
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