两个释放化物的带的细胞毒性
Seo-Rin Jeong1, Yong Kwon Chae2, Ok Hyung Nam2,3
1Department of Orthodontics, School of Dentistry, Chosun University, 61452 Gwangju, Republic of Korea.
The Journal of clinical pediatric dentistry
|September 14, 2024
概括
这项研究评估了两个释放化物的粘贴带的细胞毒性,发现在人类细胞系中,Pullulan 结合的 F-PVA 带显示出比标准 F-PVA 带更高的细胞毒性.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料 牙科材料
- 细胞生物学 细胞生物学
背景情况:
- 释放化物的带,如化聚乙醇 (NaF-PVA),是为牙科应用而开发的.
- 以前的研究表明,这些带具有抗微生物特性,并且在化物输送方面具有有效性.
- 新型化物释放带配方的细胞毒性需要在临床使用之前进行彻底的调查.
研究的目的:
- 为了评估和比较两个不同的化物释放聚乙醇 (PVA) 带的细胞毒性.
- 评估化物-PVA带在细胞毒性的pullulan合并的影响.
- 分析相关人类细胞类型中与细胞毒性和炎症反应相关的基因表达变化.
主要方法:
- 细胞毒性被评估使用3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) 对人类牙纤维细胞 (HGF) 和人类牙周带 (PDL) 细胞的测定.
- 用基因本体学 (GO) 和基因组丰富分析 (GSEA) 分析了基因稳定性和表达特征.
- 测试了两种带配方:一种标准的化PVA (F-PVA) 带和一种与普鲁兰结合的F-PVA (PF-PVA) 带.
主要成果:
- 与对照组相比,PF-PVA带的细胞毒性显著增加,对PDL细胞的效果增加66%,对HGF细胞的效果增加69%.
- 与PF-PVA带相比,F-PVA带的细胞毒性较低,PDL细胞的细胞毒性降低29%,HGF细胞的细胞毒性降低33%.
- 基因表达分析显示,与F-PVA相比,PF-PVA诱导了细胞毒性相关基因的更显著的变化,并显示了与炎症反应的更强烈关联.
结论:
- 化物-PVA粘贴带中Pullulan的结合似乎增加了它们对人类牙和牙周带细胞的细胞毒性.
- 与PF-PVA配方相比,标准的F-PVA带表现出更有利的细胞毒性概况.
- 基因表达数据支持MTT测定结果,突出了与PF-PVA带相关的潜在炎症风险,需要进一步的安全评估.
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