监测玉米炉由真菌Ustilago maydisdis进行加工
Stefan Robertz1,2, Magnus Philipp3,2,4, Kerstin Schipper3,2
1Institute for Plant Cell Biology and Biotechnology, Cluster of Excellence on Plant Sciences, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Bioresources and bioprocessing
|September 14, 2024
概括
乌斯蒂拉戈梅迪斯可以在未经处理的玉米上生长,但其对纤维素碳水化合物的使用是有限的. 提高真菌菌株和生物质消化率可以促进从农业废物中生产复合物.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 可持续的生物经济
背景情况:
- 可持续的生物经济依赖于将农业废物回收成有价值的产品.
- 玉米,玉米的副产品,是微生物生物转化的一个丰富的基质.
- 乌斯蒂拉戈梅迪斯是一种真菌生物催化剂,用于从植物生物质中生产化合物.
研究的目的:
- 调查Ustilago maydis能够利用未经处理的玉米炉作为唯一的碳来源的能力.
- 开发一个监测平台,用于玉米的真菌加工.
- 探索提高U. maydis. 菌类对纤维素利用率的方法.
主要方法:
- 在小型生物反应堆中,在未经处理的玉米上种植U. maydis.
- 在线监测真菌生长和代谢活动.
- 在发酵前后对玉米的生物化学分析.
- 与酶尾酒一起发酵,并使用工程真菌菌株.
主要成果:
- 在未经处理的玉米上,U. maydis成功地生长.
- 这种真菌主要消耗可溶性糖,其基纤维素分解有限.
- 通过提高生物质消化能力,使用酶尾酒和使用工程菌株,实现了高达120%的性能改善.
结论:
- 乌.梅迪斯可以利用玉米,但其纤维素性纤维素能力需要提高,以实现高效的生物生产.
- 优化条件和工程菌株显著改善了玉米炉的生物转化.
- 这项研究为扩大利用U. maydis.从农业废物中生产可持续化合物的基础.
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