光生物调节通过减轻氧化应激和炎症损伤以及改善线粒体功能来改善卵巢衰老
Yu He1, Rongan Ye1, Yinbo Peng1
1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Journal of photochemistry and photobiology. B, Biology
|September 14, 2024
概括
光生物调节 (PBM) 疗法可以通过改善激素水平,卵泡数量以及减少氧化应激和炎症来逆转小鼠的卵巢衰老. 这种非侵入性治疗显示出增强老化卵巢生殖健康的前景.
科学领域:
- 生殖生物学和老年学.
- 生物医学工程和光医学.
背景情况:
- 卵巢衰老带来了重大的临床挑战,有效干预措施有限.
- 光生物调制 (PBM) 是一种非侵入性疗法,有可能调节包括卵巢功能在内的生物过程.
- PBM对自然卵巢衰老的影响需要进一步研究.
研究的目的:
- 为了研究光生物调制 (PBM) 对卵巢功能在自然衰老的小鼠模型的影响.
- 探索PBM的潜在机制,包括其在氧化应激,炎症和线粒体功能中的作用.
- 在试验室中评估PBM对人类颗粒细胞 (KGN) 氧化应激的保护作用.
主要方法:
- 使用了天然卵巢衰老的小鼠模型,年轻和年长的雌性ICR小鼠接受了PBM或没有PBM的治疗 (650nm激光,4J/cm2).
- 分析了对卵巢功能,性激素水平,卵泡数量,血管生成和亡的影响.
- 在体外研究中,在用PBM治疗的KGN细胞中使用过氧化诱导的氧化应激模型.
主要成果:
- 在老年小鼠中,PBM治疗 (4 J/cm2) 显著恢复了性激素水平,增加了原始和生长的卵泡,改善了血管生成,并减少了亡.
- PBM减轻了氧化应激,慢性卵巢炎症,并在老卵巢中增强了线粒体功能.
- 在体外,PBM改善了KGN细胞活力,增殖,激素分泌,线粒体膜潜力和ATP水平,同时降低了亡和氧化应激.
结论:
- 在自然衰老的小鼠中,PBM疗法有效地恢复卵巢功能并改善与衰老相关的变化.
- 在卵巢细胞中,PBM表现出抗氧化应激的保护作用,通过抗氧化,抗炎和线粒体保护机制进行介导.
- 作为对抗卵巢衰老的治疗策略,PBM具有重大潜力.
相关概念视频
01:37Mitochondria
11.5K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
11.5K
01:46Electron Transport Chain: Complex I and II
12.2K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
12.2K
02:07Oogenesis
63.5K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.5K
01:26Aging
39
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
39
01:45Mitochondrial Membranes
9.2K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
9.2K