布法林通过激活Keap1-Nrf2/HO-1和抑制NF-κB通路来缓解实验性严重急性胰腺炎的炎症反应和氧化应激
Xiaolong Niu1, Wei Sun2, Xiaohang Tang2
1Shanghai University of Traditional Chinese Medicine, Shanghai, China; Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
International immunopharmacology
|September 14, 2024
概括
布法林通过减少炎症和氧化应激,有效地治疗大鼠的严重急性胰腺炎 (SAP). 这项研究显示了布法林.
科学领域:
- 药理学 药理学是指药理学的学科.
- 生物化学 生物化学
- 病理学 病理学 病理学
背景情况:
- 严重急性胰腺炎 (SAP) 是一种具有高死亡率的严重炎症性疾病.
- 布法林具有已知的抗炎,止痛和抗瘤特性.
- 布法林在治疗SAP中的有效性仍然未被探索.
研究的目的:
- 在SAP的老鼠模型中研究布法林的治疗潜力.
- 阐明布法林在SAP中的作用的基本机制.
主要方法:
- 在使用陶酸盐 (NaT) 的老鼠中诱导SAP.
- 血清生物化学 (氨酶,脂酶) 和胰腺病理学的评估.
- 使用ELISA,西部斑块和qPCR评估抗氧化和抗炎标志物,重点关注NF-κB和Keap1-Nrf2/HO-1通路.
主要成果:
- 布法林显著降低了血清氨酶和脂酶水平,并以剂量依赖的方式减轻了胰腺组织损伤.
- 布法林通过调节MDA,SOD,GSH和激活Keap1-Nrf2/HO-1通路来表现出强大的抗氧化作用.
- 布法林抑制了关键的炎症媒介 (TNF-α,IL-6,IL-1β) 并抑制了NF-κB信号通路.
结论:
- 布法林在NAT诱导的SAP大鼠中表现出显著的抗炎和抗氧化作用.
- 布法林的治疗作用包括抑制NF-κB和激活Keap1-Nrf2/HO-1通路.
- 布法林显示作为SAP患者的新型治疗剂具有前途.
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