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一次性,便携式电化学免疫传感器,用于在现场快速检测牛结核病

Lucia F Saad¹, Pablo A Fiorito¹, Patricia G Molina²

¹Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB) CONICET- VM-). Universidad Nacional de Villa María, Campus Universitario, Av. Arturo Jauretche 1555, C.P. X5220XAO Villa María, Córdoba, Argentina.

|September 14, 2024

概括

一个新的阻抗测量免疫传感器使用M. bovis纯化蛋白衍生物 (PPD) 检测牛结核病 (TB) 在黄金纳米粒子和聚烯. 这种具有成本效益的便携式传感器为牛的结核病诊断提供了灵敏而稳定的替代方案.

科学领域:

生物传感器是一种生物传感器.
电化学电化学电化学
兽医诊断兽医诊断兽医诊断兽医诊断

背景情况:

牛结核病 (TB) 对动物健康和全球粮食供应构成重大威胁.
目前用于牛结核病的诊断方法可能耗时,昂贵或缺乏灵敏度.
需要快速,具有成本效益和敏感的诊断工具来检测牛结核病.

研究的目的:

开发一种简单,快速,具有成本效益和灵敏的阻抗测量免疫传感器,用于在牛血清中量化牛结核病.
将M. bovis纯化蛋白衍生物 (PPD) 固定在一个修改的印电极上,用于抗体检测.
评估开发的免疫传感器的性能和稳定性.

主要方法:

在用金纳米颗粒 (AuNPs) 修改的丝网打印电极上合成聚烯 (pPy) 薄膜的电化学合成.
将M. bovis PPD固定在AuNP-pPy修饰电极上.
在牛血清样本中对抗M. bovis抗体的阻力测量检测.

主要成果:

免疫传感器显示线性检测范围从0.5μg mL-1到100μg mL-1.
对抗M. bovis抗体的检测极限 (LD) 达到了100 ng mL−1.
在牛血清样本中观察到出色的恢复率 (98%103%),具有高达30天的良好物理和化学稳定性.

结论:

开发的阻抗测量免疫传感器是一个有希望的,便携式和稳定的诊断工具,用于检测牛血清中的抗M. bovis抗体.
它比结核素和ELISA等传统方法具有优势,包括直接测量,最小的样本体积和避免有害试剂.
这项技术促进了大规模检测,并作为牛结核病诊断的可行替代方案.

关键词:

牛结核是牛结核的一种疾病.电化学阻抗光谱学电化学阻抗光谱学黄金纳米颗粒的使用方法免疫传感器是一种免疫传感器.聚乙烯 (Polypyrrole) 是一种聚乙烯 (Polypyrrole) 的一种.

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