黄皮醇抑制了卵细胞的成熟,降低了卵细胞的质量,并阻断了累积细胞的功能
Xiao-Zhen Li1, Li-Tao Yi2, Qing-Yuan Sun2
1College of Life Sciences, Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China; Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou 510317, China.
Toxicology letters
|September 14, 2024
概括
黄皮醇 (FP) 通过抑制卵细胞成熟和引起细胞亡,对女性生育能力产生负面影响. 这种药物可能会导致生殖系统的副作用,在癌症治疗期间需要仔细考虑.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 癌症药理学 癌症药理学
背景情况:
- 黄皮醇 (FP) 是一种植物性黄类化合物,用于治疗癌症,真菌和炎症.
- FP对女性生殖系统的潜在副作用尚未得到充分了解.
研究的目的:
- 研究FP对小鼠卵细胞成熟和累积细胞扩张的毒性影响.
- 阐明FP诱导的生殖毒性的潜在机制.
主要方法:
- 在体外培养具有不同FP度 (50-1000nM) 的累积卵细胞复合体 (COC).
- 评估卵细胞成熟率和累积膨胀指数.
- 使用了免疫光染色,qRT-PCR和卵细胞染色体制备.
主要成果:
- 暴露于FP以剂量依赖的方式抑制了卵细胞成熟和累积细胞扩张.
- 在COC中,FP诱导了亡.
- 高度的FP (500nM) 引起了螺旋和染色体异常,导致成熟停止和动质化.
结论:
- 黄二醇对女性生殖细胞具有剂量依赖的毒性.
- 氧化应激,线粒体功能障碍和错位是FP不良影响背后的潜在机制.
- 在临床使用过程中,FP对女性生育能力的潜在影响需要认真考虑.
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