产生大豆蛋白分离物的新方法:使用微生物固态发酵产生的蛋白酶,对高温大豆粉进行直接的酶性水解
Junsong Zhu1, Dandan Liu1, Feng Lu2
1School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
International journal of biological macromolecules
|September 14, 2024
概括
这项研究引入了一种使用发酵大豆粉提取大豆蛋白分离物 (SPI) 的新方法. 这种发酵过程显著提高了SPI产量,并提高了其氨基酸含量,提供了更有效的提取技术.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物技术是生物技术.
- 微生物学 微生物学
背景情况:
- 传统的大豆蛋白分离物 (SPI) 提取方法在产量和效率方面面临挑战,特别是从经过高温处理的大豆粉中.
- 微生物发酵为提高大豆蛋白的提取性和营养特征提供了一个潜在的途径.
研究的目的:
- 开发和评估一种新的固态发酵方法,以提高从高温大豆粉中提取大豆蛋白分离物的效果.
- 优化发酵条件并评估Bacillus amyloliquefaciens亚种的影响. 植物 CICC 10265 关于 SPI 的产量和质量.
主要方法:
- 使用Bacillus amyloliquefaciens亚种的高温大豆粉的固体发酵. 脚部 CICC 10265.5. 脚部 CICC 10265.5. 脚部 CICC 10265.5. 脚部 CICC 10265.5. 脚部 CICC 10265. 脚部 CICC 10265.5. 脚部 CICC 10265. 脚部 CICC 10265.
- 在发酵过程中监测蛋白酶活性和微生物生长,以确定最佳提取时间.
- 从发酵和未发酵的大豆粉中提取大豆蛋白分离物 (SPI),进行比较分析.
主要成果:
- 用Bacillus amyloliquefaciens亚种进行固体发酵. 足部 CICC 10265 与直接提取相比,SPI提取产量明显提高了130.19%.
- 在发酵8小时后,获得了最佳的SPI提取,平衡了蛋白酶活性和微生物生长.
- 从发酵大豆粉中提取的SPI显示总氨基酸含量高出2.1%,并符合微生物安全标准.
结论:
- 百菌氨基面菌子物种. plantarum CICC 10265对于大豆粉的固态发酵是有效的,增强了SPI提取.
- 新的发酵辅助提取方法可以大幅增加SPI产量并改善营养价值.
- 优化的发酵协议可以导致更高效和更高质量的大豆蛋白分离物生产.
相关概念视频
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Production of Pharmaceuticals
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...
Microbial Bioremediation of Plastics
Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...


