使用低放大光显微镜对大肠杆菌和沙门氏菌的光学检测和计数
Yuzhen Zhang1, Zili Gao1, Lili He2
1Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
Journal of microbiological methods
|September 14, 2024
概括
一种新的光学检测方法使用一块金芯片涂上3 - 墨甲酸 (3-MPBA),用于快速检测细菌细胞. 这种具有成本效益的技术允许使用标准光显微镜对表面上的细菌进行量化,这对于食品安全和卫生至关重要.
科学领域:
- 分析化学 分析化学
- 生物感应是一种生物感应.
- 表面科学是一门学科.
背景情况:
- 快速和经济有效的细菌检测对于食品安全,临床卫生和制药质量控制至关重要.
- 目前用于表面细菌检测的方法可能耗时,昂贵或需要专门的设备.
研究的目的:
- 建立一个快速,经济高效的光学检测方法,用于表面上的细菌细胞.
- 将这种方法与抽样采样相结合,以便在评估表面污染时实际应用.
- 为了使用标准光显微镜进行细菌量化.
主要方法:
- 开发了一种金芯片传感器,涂上3 - 默卡普托菲尼尔酸 (3-MPBA),用于捕获细菌细胞.
- 使用标准光显微镜与10×目标镜头用于低放大检测和量化.
- 整合了光学检测方法与抽样采样进行表面分析.
- 使用热处理来增强对特定细菌物种的敏感性.
主要成果:
- 在103 CFU/mL (分别为76.0%和81.1%) 达到高捕获沙门氏体 (SE1045) 和大肠杆菌 (E. coli OP50) 的高捕获效率.
- 细菌度和细胞计数之间的良好线性关系在103-107 CFU/mL (SE1045) 和103-108 CFU/mL (大肠杆菌OP50) 之间得到证明.
- 确定了两种细菌的检测极限 (LOD) 为103 CFU/mL,通过热处理将大肠杆菌OP50降低到102 CFU/mL.
- 成功评估了不钢表面的细菌污染,回收率为~40%.
结论:
- 开发的光学检测方法是快速的 (约. 2小时),具有成本效益 (每样数美元),适合在表面进行细菌量化.
- 该方法显示了在细菌污染控制中的工业应用的巨大潜力,特别是在资源不足的环境中.
- 与拭子采样的整合和提高灵敏度的可能性为表面卫生监测提供了一个多功能工具.
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