德布可纳会加剧抗生素耐药性基因的同时发生和水平转移
Yuanfei Gao1, Yuchen Guo1, Lanjun Wang1
1National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Tai'an 271018, People's Republic of China.
Pesticide biochemistry and physiology
|September 14, 2024
概括
暴露于德布可纳真菌杀菌剂显著增加了抗生素耐药性基因 (ARG) 在土壤生态系统中的水平转移. 这种杀虫剂通过增强细菌生物膜形成和细胞膜透性来促进ARG的传播.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 遗传学 是一个遗传学.
背景情况:
- 铁布可纳是一种广泛使用的在土壤中发现的杀菌剂.
- 抗生素耐药性基因 (ARG) 在土壤环境中也很普遍.
- 德布可纳对ARG水平转移的影响在很大程度上是未知的.
研究的目的:
- 为了调查tebuconazole对ARG丰富度和在土壤中的水平转移的影响.
- 阐明tebuconazole影响ARG转移的机制.
主要方法:
- 模拟的铁布可纳污染的土壤生态系统.
- 等离子体RP4介导的结合转移系统.
- 扫描电子显微镜,流细胞计和基因表达分析.
主要成果:
- 德布可纳暴露增加了7.93倍的等离子体结合转移频率.
- 德布可纳促进了生物膜的形成,并增加了细胞膜的透性.
- 对 ompA,氧化应激和SOS响应基因的升级与传输频率相关.
结论:
- 德布可纳作为选择性压力作用,显著促进了ARGs的水平转移.
- 生物膜形成和细胞膜透性的改变是关键机制.
- 这突显了农药使用对抗生素耐药性传播的潜在环境风险.
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